| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Teixeira, Anthony J (16)
|
NISKAN |
32.07 |
34.11 |
2.04 |
| 2 |
Keith, Bradley N (21)
|
NIISWM |
38.00 |
37.55 |
-0.45 |
| 3 |
Warner, Tyler J (14)
|
NIMETS |
48.77 |
40.69 |
-8.08 |
| 4 |
Mueller, Christopher J (15)
|
NISDSC |
48.87 |
40.96 |
-7.91 |
| 5 |
Creed, Jason M (14)
|
NIUN-0 |
43.81 |
41.24 |
-2.57 |
| 6 |
Williams, Jonah F (12)
|
NISKAN |
38.74 |
41.26 |
2.52 |
| 7 |
El Ghissassi, Awab (11)
|
NIVEST |
41.67 |
42.00 |
0.33 |
| 8 |
Olsian, Jack J (14)
|
NIUNAC |
47.63 |
44.34 |
-3.29 |
| 9 |
Cole, Cullin F (13)
|
NIKRKN |
|
45.01 |
- |
| 10 |
Peters, Evan M (12)
|
NIGCOM |
|
45.40 |
- |
| 11 |
Naftel, Norbusamten A (15)
|
NIISWM |
45.00 |
45.43 |
0.43 |
| 12 |
Cocco, Nicholas A (13)
|
NIVEST |
51.45 |
45.50 |
-5.95 |
| 13 |
Mills, William P (12)
|
NIMETS |
|
46.55 |
- |
| 14 |
Attuwaybi, Hamza B (10)
|
NIVELO |
46.11 |
47.29 |
1.18 |
| 15 |
Nelson, Payton J (13)
|
NIMETS |
57.64 |
47.41 |
-10.23 |
| 16 |
TenEyck, Kaden T (12)
|
NIKRKN |
|
49.90 |
- |
| 17 |
Marino, Luke A (13)
|
NISYCH |
50.00 |
50.59 |
0.59 |
| 18 |
Lemmo, Nicholas D (11)
|
NIVELO |
52.04 |
51.48 |
-0.56 |
| 19 |
Podoliak, Kaleb A (11)
|
NIKRKN |
51.30 |
51.66 |
0.36 |
| 20 |
Refici, Nathan P (13)
|
NISDSC |
|
53.49 |
- |
| 21 |
Harris, Wesley S (9)
|
NIVICT |
|
58.78 |
- |
| 22 |
Ratteree, Jackson R (11)
|
NIVEST |
58.41 |
1:00.18 |
1.77 |
| 23 |
Worob, Jack H (10)
|
NIISWM |
1:00.88 |
1:01.91 |
1.03 |
| 24 |
Ramsperger, Jacob T (10)
|
NISDSC |
|
1:07.33 |
- |
| 25 |
Refici, Zane S (9)
|
NISDSC |
|
1:10.94 |
- |
| 26 |
Warner, Lucas M (8)
|
NIMETS |
1:27.23 |
1:15.99 |
-11.24 |
| 27 |
Ashby, Colton J (7)
|
NISDSC |
|
1:17.97 |
- |
| 28 |
Knausz, Imre (8)
|
NISDSC |
|
2:10.66 |
- |
|
Mitsakos, Max A (10)
|
NIUNAC |
1:09.65 |
DQ |
- |
|
Panol, Alexander T (12)
|
NISYCH |
|
NS |
- |