| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nalli, Alexia C (10)
|
MABLUE |
30.23 |
32.11 |
1.88 |
| 2 |
Harmon, Sophia D (10)
|
MAEMAC |
34.30 |
33.92 |
-0.38 |
| 3 |
McGlynn, Shea (10)
|
MABLUE |
33.75 |
34.31 |
0.56 |
| 4 |
Murphy, Ciara J (10)
|
MAKIDS |
36.16 |
37.18 |
1.02 |
| 5 |
Newhard, Kate E (9)
|
MAEMAC |
36.46 |
38.15 |
1.69 |
| 6 |
Walp, Maliya H (9)
|
MAKIDS |
36.57 |
40.72 |
4.15 |
| 7 |
Evans, Tegan R (9)
|
MAPAAC |
41.28 |
41.26 |
-0.02 |
| 8 |
Newhart, Reese E (9)
|
MAEMAC |
47.98 |
44.05 |
-3.93 |
| 9 |
Buglino, Kaylee A (9)
|
MALVA |
45.17 |
46.79 |
1.62 |
| 10 |
Shannon, Lia M (9)
|
MABLUE |
44.61 |
48.07 |
3.46 |
| 11 |
Froetschner, Avelyn C (9)
|
MAKIDS |
|
48.47 |
- |
| 12 |
Kiss, Katelyn (9)
|
MAKIDS |
49.46 |
51.37 |
1.91 |
| 13 |
Fastenberg, Christina N (10)
|
MAPAAC |
46.31 |
1:03.65 |
17.34 |