| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Salmassian, Christine K (8)
|
NTDM |
38.04 |
38.32 |
0.28 |
| 2 |
Euseppi, Gia L (8)
|
NTDM |
44.93 |
44.41 |
-0.52 |
| 3 |
HA, Nlha (8)
|
NTPSC |
|
44.66 |
- |
| 4 |
Flaming, Gianna E (7)
|
NTMTRO |
1:03.46 |
47.50 |
-15.96 |
| 5 |
Sears, Ainslee K (7)
|
NTCOPS |
51.45 |
49.74 |
-1.71 |
| 6 |
Bigenho Blackburn, Lillian G (8)
|
NTLTSD |
51.19 |
50.59 |
-0.60 |
| 7 |
Vijee, Reshikaa (8)
|
NTCOPS |
|
50.93 |
- |
| 8 |
Holladay, Hallie A (6)
|
NTATAC |
|
52.50 |
- |
| 9 |
Wilson, Elyse H (8)
|
NTJMSS |
55.02 |
52.60 |
-2.42 |
| 10 |
Fitzgerald, Taryn S (8)
|
NTCOPS |
52.49 |
53.52 |
1.03 |
| 11 |
Salmassian, Catherine E (6)
|
NTDM |
52.68 |
54.22 |
1.54 |
| 12 |
Perez, Brianna C (8)
|
NTSTAR |
1:01.46 |
54.32 |
-7.14 |
| 13 |
Barker, Katherine M (8)
|
NTUN |
59.45 |
55.03 |
-4.42 |
| 14 |
Perez, Bianca D (7)
|
NTSTAR |
|
56.95 |
- |
| 15 |
Pacha, Hannah N (7)
|
NTDM |
|
57.11 |
- |
| 16 |
Howza, Elle R (8)
|
NTMTRO |
1:03.85 |
57.88 |
-5.97 |
| 17 |
Persefield, Abigail E (8)
|
NTATAC |
55.88 |
58.49 |
2.61 |
| 18 |
Sullivan, Gracie L (8)
|
NTLTSD |
1:02.34 |
1:00.60 |
-1.74 |
| 19 |
Kaden, Kinsler C (7)
|
NTATAC |
|
1:01.59 |
- |
| 20 |
Springborn, Molly L (8)
|
NTDM |
57.00 |
1:03.62 |
6.62 |
| 21 |
Fiderfish, Elizabeth V (8)
|
NTLTSD |
|
1:03.91 |
- |
| 22 |
Vijey, Hira (8)
|
NTLTSD |
1:08.11 |
1:04.17 |
-3.94 |
| 23 |
Shaw, Vivien A (8)
|
NTLTSD |
|
1:05.66 |
- |
|
Martinez, Jennifer E (8)
|
NTMTRO |
|
DQ |
- |
|
Coats-Lenling, Asher B (6)
|
NTATAC |
|
NS |
- |
|
Peck, Zoe L (8)
|
NTESP |
|
NS |
- |