| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lakatos Alcantara, Lucia E (10)
|
PVNCAP |
49.16 |
46.41 |
-2.75 |
| 2 |
Walther, Rio S (9)
|
PVNCAP |
49.68 |
46.52 |
-3.16 |
| 3 |
Lay, Kamryn J (9)
|
PVMACH |
48.33 |
47.56 |
-0.77 |
| 4 |
Pezzimenti, Natalia M (9)
|
PVMACH |
47.87 |
48.07 |
0.20 |
| 5 |
Clark, Keegan E (10)
|
PVNCAP |
46.41 |
48.26 |
1.85 |
| 6 |
Showalter, Elle (9)
|
PVMACH |
51.04 |
48.79 |
-2.25 |
| 7 |
Lasut, Claire I (10)
|
PVMACH |
49.36 |
50.32 |
0.96 |
| 8 |
Hirsch, Mikayla B (10)
|
PVNCAP |
50.32 |
50.93 |
0.61 |
| 9 |
Shoemaker, Madeline G (9)
|
PVNCAP |
50.87 |
51.12 |
0.25 |
| 10 |
Budd, Genevieve A (10)
|
PVNCAP |
54.14 |
54.53 |
0.39 |
| 11 |
Duffy, Susannah G (10)
|
PVNCAP |
55.19 |
55.28 |
0.09 |
| 12 |
Onerci, Gizem (9)
|
PVMACH |
57.30 |
55.44 |
-1.86 |
| 13 |
Palmer, Brielle A (10)
|
PVMACH |
1:01.42 |
57.62 |
-3.80 |
| 14 |
Redburn, Tara (9)
|
PVNCAP |
59.05 |
1:11.05 |
12.00 |
|
Frantz, Graycen M (9)
|
PVMACH |
1:14.38 |
DQ |
- |
|
Tolar, Mary M (9)
|
PVNCAP |
56.84 |
DQ |
- |
|
Gomez Crummett, Candela N (10)
|
PVNCAP |
57.44 |
NS |
- |