| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Krenn, Kendall R (10)
|
AMSRST |
2:25.72 |
2:38.10 |
12.38 |
| 2 |
Acerra, Shannon M (10)
|
AMHD |
2:49.28 |
2:46.75 |
-2.53 |
| 3 |
Schork, Samantha N (10)
|
AMGPAC |
2:49.49 |
2:48.11 |
-1.38 |
| 4 |
Werner, Ayla R (10)
|
AMNHA |
2:56.77 |
2:59.53 |
2.76 |
| 5 |
Rupert, Rachel L (9)
|
AMPRA |
3:02.65 |
3:02.91 |
0.26 |
| 6 |
Peretti, Cameron S (10)
|
AMWHAT |
3:27.34 |
3:23.44 |
-3.90 |
| 7 |
Moore, Olivia S (10)
|
AMHD |
3:51.98 |
3:25.92 |
-26.06 |
| 8 |
Schomaker, Charlotte J (7)
|
AMNHA |
3:59.66 |
3:27.70 |
-31.96 |
| 9 |
Hertzig, Giavannah M (10)
|
AMPRA |
3:49.04 |
3:28.44 |
-20.60 |
| 10 |
DeMarco, Isabelle R (10)
|
AMSRST |
3:50.23 |
3:41.57 |
-8.66 |
| 11 |
Rees, Leia K (7)
|
AMNHA |
4:01.66 |
3:42.77 |
-18.89 |
|
Biltz, Avery M (10)
|
AMCSC |
2:36.41 |
NS |
- |