| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Romanoff, Claire J (7)
|
AMPEAQ |
21.92 |
22.47 |
0.55 |
| 2 |
Roethlisberger, Baylee M (7)
|
AMSTEE |
27.10 |
23.99 |
-3.11 |
| 3 |
Rossman, Alexia H (7)
|
AMRXA |
25.37 |
25.55 |
0.18 |
| 4 |
Watkins, Natalie A (7)
|
AMBPR |
45.13 |
25.61 |
-19.52 |
| 5 |
Permar, Aloisa R (7)
|
AMSTEE |
26.37 |
25.72 |
-0.65 |
| 6 |
Kunkle, Heather M (7)
|
AMPTAC |
27.96 |
25.79 |
-2.17 |
| 7 |
Dudro, Eleanor P (7)
|
AMANSC |
26.19 |
26.75 |
0.56 |
| 8 |
Tomaino, Olivia A (7)
|
AMHD |
30.32 |
27.11 |
-3.21 |
| 9 |
Empfield, Danielle C (7)
|
AM3RVS |
29.19 |
28.70 |
-0.49 |
| 10 |
Quinn, Ava V (7)
|
AMSASC |
28.87 |
29.06 |
0.19 |
| 11 |
Crowley, Charlotte M (7)
|
AMBPR |
38.17 |
29.25 |
-8.92 |
| 12 |
Hartle, Lilee M (7)
|
AM3RVS |
31.96 |
30.39 |
-1.57 |
| 13 |
Sincraugh, Payton R (7)
|
AMPEAQ |
36.36 |
30.49 |
-5.87 |
| 14 |
Silvaggio, Josephine M (7)
|
AMPRA |
33.87 |
31.15 |
-2.72 |
| 15 |
Tropeck, Samantha K (7)
|
AMWAAC |
31.13 |
31.41 |
0.28 |
| 16 |
Veldkamp, Claire A (7)
|
AMHD |
34.69 |
32.55 |
-2.14 |
| 17 |
Ward, Coraline V (7)
|
AMMMSA |
32.76 |
34.55 |
1.79 |
| 18 |
Shroyer, Kadence J (7)
|
AMWAAC |
29.03 |
34.93 |
5.90 |
|
Weber, Margot G (7)
|
AMFAST |
27.14 |
DQ |
- |
|
Scott, Blair M (7)
|
AMKW |
24.94 |
DQ |
- |