| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spano, Molly K (17)
|
GUUN-S |
5:17.41 |
5:32.27 |
14.86 |
| 2 |
Barton, Samantha V (15)
|
GUPFL |
5:36.90 |
5:37.85 |
0.95 |
| 3 |
Torres, Celia G (18)
|
GUPACE |
5:33.92 |
5:50.94 |
17.02 |
| 4 |
Berkman, Kaylie J (17)
|
GUPFL |
6:00.00 |
5:51.42 |
-8.58 |
| 5 |
Reitenour, Danielle N (15)
|
GUBLST |
5:42.12 |
5:58.55 |
16.43 |
| 6 |
Perez, Gina R (16)
|
GUPFL |
6:00.08 |
6:02.18 |
2.10 |
| 7 |
Watts, Emma F (15)
|
GUPFL |
|
6:04.30 |
- |
| 8 |
Noonan, Miranda B (15)
|
GUBATS |
5:48.05 |
6:05.26 |
17.21 |
| 9 |
Lopez, Catalina (15)
|
GUBLST |
6:12.59 |
6:17.07 |
4.48 |
| 10 |
Reyes, Grace N (15)
|
GUBLST |
|
6:19.27 |
- |
| 11 |
Willis, Alyssa A (15)
|
GUPLAT |
7:08.23 |
6:20.07 |
-48.16 |
| 12 |
Green, Emily E (15)
|
GUPACE |
6:45.87 |
6:27.62 |
-18.25 |
| 13 |
Bourgeois-Solomon, Sovereign (15)
|
GUPACE |
6:22.13 |
6:34.52 |
12.39 |
| 14 |
Brosch, Kaylee G (16)
|
GUPFL |
6:35.37 |
6:36.62 |
1.25 |