| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vu, Grant H (10)
|
MDCAA |
34.81 |
34.75 |
-0.06 |
| 2 |
Giza, Brody R (10)
|
MDFSC |
37.18 |
35.95 |
-1.23 |
| 3 |
VerMerris, Carter P (9)
|
MDNAAC |
37.97 |
36.06 |
-1.91 |
| 4 |
Tom, Matthew S (10)
|
MDCAA |
36.06 |
36.39 |
0.33 |
| 5 |
Smith, Jackson T (10)
|
MDFSC |
37.91 |
36.77 |
-1.14 |
| 6 |
Li, Harvey (10)
|
MDCAA |
36.79 |
37.31 |
0.52 |
| 7 |
Jimenez, Yan F (9)
|
MDASC |
39.68 |
40.11 |
0.43 |
| 8 |
Janusziewicz, Mason L (10)
|
MDFSC |
41.48 |
41.03 |
-0.45 |
| 9 |
Sonecha, Reid M (10)
|
MDASC |
43.49 |
42.38 |
-1.11 |
| 10 |
Xu, Maverick B (9)
|
MDCAA |
45.40 |
43.44 |
-1.96 |
| 11 |
Wayman, Wyatt M (10)
|
MDASC |
48.18 |
44.16 |
-4.02 |
| 12 |
Bettis, Henry T (10)
|
MDFSC |
49.46 |
44.48 |
-4.98 |
| 13 |
Emrick, Ilya P (10)
|
MDFAST |
44.07 |
46.66 |
2.59 |
| 14 |
Cannone, Charles W (10)
|
MDASC |
|
49.16 |
- |
| 15 |
Pennington, Christian H (9)
|
MDASC |
|
50.83 |
- |
|
Kalanzi, Enoch A (10)
|
MDASC |
47.39 |
DQ |
- |
|
Figueroa-Chabrier, Sebastian (10)
|
MDFSC |
35.99 |
NS |
- |