| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roybal, Hudson L (12)
|
NTLAC |
55.02 |
55.03 |
0.01 |
| 2 |
Nguyen, Philippe D (12)
|
NTCOPS |
55.97 |
55.73 |
-0.24 |
| 3 |
Beck, Jackson E (12)
|
NTLAC |
56.27 |
56.00 |
-0.27 |
| 4 |
Landry, Zavin L (12)
|
NTNTN |
56.48 |
56.08 |
-0.40 |
| 5 |
Pan-Wang, Evan (11)
|
NTLAC |
57.18 |
56.38 |
-0.80 |
| 6 |
Hunt, Brendan B (12)
|
NTTFA |
57.43 |
57.58 |
0.15 |
| 7 |
Isaacs-Sodeye, Judah I (12)
|
NTNTN |
59.59 |
57.59 |
-2.00 |
| 8 |
Ruhl, Jonah W (11)
|
NTATAC |
59.46 |
58.28 |
-1.18 |
| 9 |
Heldenbrand, Dylan F (12)
|
NTNTN |
57.93 |
59.17 |
1.24 |
| 10 |
Merrill, Elijah R (12)
|
NTRACE |
58.65 |
59.86 |
1.21 |
| 11 |
Dickheiser, Kobe Y (11)
|
NTNTN |
1:01.09 |
1:00.18 |
-0.91 |
| 12 |
Schnapp, Logan N (11)
|
NTLAC |
1:02.86 |
1:01.06 |
-1.80 |
| 13 |
Lofthus, Jack W (11)
|
NTLAC |
1:02.39 |
1:02.39 |
0.00 |
| 14 |
Duran De La Vega, Pablo (12)
|
NTMTRO |
1:00.79 |
1:02.82 |
2.03 |
| 15 |
Phillips, Hudson T (11)
|
NTTFA |
1:03.12 |
1:05.30 |
2.18 |