| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Petric, Luke S (12)
|
NTLIFE |
38.52 |
34.59 |
-3.93 |
| 2 |
Tobin, Michael I (12)
|
NTATAC |
35.93 |
34.98 |
-0.95 |
| 3 |
Topolewski, Lucas S (11)
|
NTMAC |
36.06 |
35.47 |
-0.59 |
| 4 |
Zhang, Yuanben (11)
|
NTLIFE |
35.35 |
36.43 |
1.08 |
| 5 |
Abshire, Andrew (11)
|
NTATAC |
41.74 |
39.48 |
-2.26 |
| 6 |
Johnson, Grant M (11)
|
NTLIFE |
|
39.93 |
- |
| 7 |
Tuapen, Angelo Jacob V (11)
|
NTNTN |
39.59 |
40.44 |
0.85 |
| 8 |
Stubblefield, Lucas S (12)
|
NTMAC |
40.53 |
40.78 |
0.25 |
| 9 |
Mazidi, Ethan A (12)
|
NTMAC |
45.68 |
40.85 |
-4.83 |
| 10 |
Sandifer, Carson B (11)
|
NTLIFE |
|
46.22 |
- |
| 11 |
Llanas Jr., Homero (11)
|
NTMARS |
53.35 |
47.72 |
-5.63 |
| 12 |
Ford, Mason A (12)
|
NTMAC |
51.44 |
47.75 |
-3.69 |
| 13 |
Shaffner, Logan D (11)
|
NTCOR |
51.11 |
48.08 |
-3.03 |
| 14 |
Ralls, Mykah A (12)
|
NTMARS |
|
48.75 |
- |
| 15 |
Serralta, Gavin W (11)
|
NTMAC |
|
56.58 |
- |
| 16 |
Campbell, Aidan T (11)
|
NTCOR |
55.32 |
58.28 |
2.96 |
| 17 |
Murray, Luke V (11)
|
NTCOR |
1:00.35 |
59.84 |
-0.51 |
|
Guzman, Angel G (12)
|
NTLIFE |
1:02.52 |
DQ |
- |
|
Badura, Adam J (12)
|
NTLIFE |
|
DQ |
- |
|
Villarreal, Kyler R (12)
|
NTLIFE |
|
NS |
- |