| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zettler, Grace A (6)
|
PVASA |
25.51 |
23.01 |
-2.50 |
| 2 |
Ter, Olivia E (6)
|
PVNCAP |
26.05 |
24.97 |
-1.08 |
| 3 |
Minear, Abigail C (6)
|
PVAAC |
26.70 |
26.07 |
-0.63 |
| 4 |
van de Graaff, Alexis L (6)
|
PVMAKO |
28.20 |
27.62 |
-0.58 |
| 5 |
Yao, Catherine J (6)
|
PVFISH |
29.54 |
29.57 |
0.03 |
| 6 |
Dominski, Norah J (6)
|
PVFBST |
29.81 |
30.14 |
0.33 |
| 7 |
Dussault, Eva P (6)
|
PVSDS |
33.31 |
31.31 |
-2.00 |
| 8 |
Close, Kaelin M (6)
|
PVAAC |
30.44 |
31.87 |
1.43 |
| 9 |
Lehman, Ainsley B (6)
|
PVSDS |
31.36 |
34.24 |
2.88 |
| 10 |
Hastings, Jane C (6)
|
PVNCAP |
37.83 |
34.92 |
-2.91 |
| 11 |
Wiley-Jimenez, Ariadne M (6)
|
PVFISH |
32.65 |
35.72 |
3.07 |
| 12 |
Shumway, Sabrina L (6)
|
PVHACC |
|
38.57 |
- |
|
Brower, Sutton L (6)
|
PVAAC |
37.06 |
NS |
- |
|
Cao, April A (6)
|
PVAAC |
39.55 |
NS |
- |
|
Reilly, Clara G (6)
|
PVSDS |
28.61 |
NS |
- |