| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
French, Raven A (10)
|
ADBENN |
43.04 |
42.33 |
-0.71 |
| 2 |
Young, Julia T (9)
|
ADTAC |
46.30 |
44.50 |
-1.80 |
| 3 |
Lamb, Vivienne E (9)
|
ADTAC |
43.66 |
45.33 |
1.67 |
| 4 |
Vroman, Ava G (10)
|
ADCANA |
44.35 |
45.34 |
0.99 |
| 5 |
Meka, Olivia G (10)
|
ADCANA |
40.91 |
45.51 |
4.60 |
| 6 |
Roos, Avada L (10)
|
ADCANA |
43.99 |
46.84 |
2.85 |
| 7 |
Perri, Gabriella M (9)
|
ADDELM |
43.59 |
47.21 |
3.62 |
| 8 |
Blaum, Sarah T (9)
|
ADBSPA |
49.79 |
50.61 |
0.82 |
| 9 |
McFarland, Charlotte Q (9)
|
ADTAC |
|
56.47 |
- |
| 10 |
Robles, Isabella M (9)
|
ADDELM |
53.52 |
58.22 |
4.70 |
| 11 |
Sokolewicz, Catherine N (10)
|
ADBSPA |
|
1:05.15 |
- |
| 12 |
Bitteker, Greta A (10)
|
ADBENN |
|
1:12.43 |
- |
| 13 |
Daley, Emma M (9)
|
ADBENN |
|
1:13.30 |
- |
|
Frew, Emery P (10)
|
ADCANA |
1:04.27 |
DQ |
- |
|
Beckenstein, Sierra L (10)
|
ADBSPA |
|
DQ |
- |
|
Rajulu, Hasni K (10)
|
ADDELM |
|
DQ |
- |
|
Rajulu, Maegna k (10)
|
ADDELM |
|
DQ |
- |
|
Martynyuk, Kajsa J (10)
|
ADBSPA |
1:01.19 |
DQ |
- |
|
Salvesvold, Ava A (10)
|
ADBENN |
|
NS |
- |
|
Cronlund, Miranda M (10)
|
ADDELM |
42.55 |
NS |
- |
|
Fiato, Nora R (9)
|
ADJJSC |
|
NS |
- |