| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ritterhoff, Anna K (16)
|
MDMAS |
59.19 |
59.88 |
0.69 |
| 2 |
Farina, Isabella M (15)
|
MDMAS |
1:01.80 |
59.96 |
-1.84 |
| 3 |
Shener, Alison M (15)
|
MDLBA |
59.45 |
1:01.25 |
1.80 |
| 4 |
Csiki, Sophie A (17)
|
MDYCM |
1:01.09 |
1:01.33 |
0.24 |
| 5 |
Perigo, Kiera M (15)
|
MDEST |
1:00.69 |
1:02.33 |
1.64 |
| 6 |
Reid, Peyton S (15)
|
MDMAS |
1:04.47 |
1:03.06 |
-1.41 |
| 7 |
Kelley, Fiona M (17)
|
MDSPRC |
1:03.68 |
1:03.51 |
-0.17 |
| 8 |
Feng, Sophia R (15)
|
MDMAS |
1:04.11 |
1:03.80 |
-0.31 |
| 9 |
Marshall, Helen K (16)
|
MDLBA |
1:01.00 |
1:04.34 |
3.34 |
| 10 |
Sheinin, Lucinda L (17)
|
MDLBA |
59.48 |
1:05.61 |
6.13 |
| 11 |
Hittle, Ava E (15)
|
MDEST |
1:05.89 |
1:06.39 |
0.50 |
| 12 |
Harding, Annaliese J (17)
|
MDSPRC |
1:03.48 |
1:06.61 |
3.13 |
| 13 |
Charles, Madison O (15)
|
MDMAS |
1:06.15 |
1:07.74 |
1.59 |
| 14 |
Everitt, Emily C (16)
|
MDYCM |
1:09.56 |
1:10.54 |
0.98 |
| 15 |
Puleo, Eva M (15)
|
MDMAS |
1:09.05 |
1:11.19 |
2.14 |
| 16 |
Dick, Sydney M (15)
|
MDMAS |
1:09.56 |
1:12.21 |
2.65 |
| 17 |
Powell, Ania L (16)
|
MDFMSC |
1:12.51 |
1:16.04 |
3.53 |
| 18 |
Wilderson, Breanna L (16)
|
MDYCM |
1:12.82 |
1:16.54 |
3.72 |
| 19 |
Morosi, Camilla (16)
|
MDYCM |
1:19.49 |
1:16.56 |
-2.93 |
| 20 |
LaDana, Juliana (20)
|
MDYCM |
1:22.04 |
1:23.83 |
1.79 |
|
Everhart, Makayla G (17)
|
MDSPRC |
1:05.59 |
NS |
- |
|
Zeller, Natalie P (16)
|
MDMAS |
1:07.98 |
NS |
- |