| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hughes, Quinn (8)
|
MDYCM |
|
47.39 |
- |
| 2 |
Johnston, Mabel E (8)
|
MDMBK |
48.80 |
47.78 |
-1.02 |
| 3 |
Groves, Kinsley A (8)
|
MDMAS |
54.88 |
51.72 |
-3.16 |
| 4 |
Morse, Abigail V (8)
|
MDEST |
|
58.71 |
- |
| 5 |
Wolfe, Dylan C (8)
|
MDMAS |
1:03.33 |
58.83 |
-4.50 |
| 6 |
Amoah, Veliane K (8)
|
MDYCM |
|
59.14 |
- |
| 7 |
Guy, Nadia R (8)
|
MDYCM |
|
1:01.26 |
- |
| 8 |
Decktor, Aurora Q (7)
|
MDEST |
|
1:04.79 |
- |
| 9 |
Malone, Madelyn A (7)
|
MDACA |
|
1:06.19 |
- |
| 10 |
Knight, Lucy A (7)
|
MDEST |
|
1:08.31 |
- |
| 11 |
Wenzel, Scarlett A (7)
|
MDMAS |
|
1:10.39 |
- |
|
Gorugantula, Sreshta (8)
|
MDGBSA |
|
DQ |
- |
|
Schindler, Julianna J (7)
|
MDGTAC |
|
DQ |
- |
|
Richmond, Alexis L (8)
|
MDYCM |
|
DQ |
- |