| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stokes, Kennedy A (10)
|
MDUN |
44.59 |
41.95 |
-2.64 |
| 2 |
Cason, Ameenah B (9)
|
MDBCSC |
1:13.21 |
47.86 |
-25.35 |
| 3 |
Connor, Ashley E (9)
|
MDASC |
45.43 |
47.93 |
2.50 |
| 4 |
Pierce, Laci M (10)
|
MDACA |
56.83 |
48.06 |
-8.77 |
| 5 |
Wu, Sunny (9)
|
MDCAA |
|
48.20 |
- |
| 6 |
Harner, Trista J (10)
|
MDOPST |
42.49 |
48.56 |
6.07 |
| 7 |
Churilla, Reese H (10)
|
MDCAA |
53.59 |
48.94 |
-4.65 |
| 8 |
Swope, Natalie R (10)
|
MDASC |
41.92 |
49.70 |
7.78 |
| 9 |
Cheer, Cora (9)
|
MDCAA |
52.53 |
50.83 |
-1.70 |
| 10 |
Hartnett, Shannen M (9)
|
MDASC |
46.41 |
51.10 |
4.69 |
| 11 |
Cahill, Kimberly R (9)
|
MDYRAC |
57.26 |
53.98 |
-3.28 |
| 12 |
Pangan, Giuliana Darlene P (10)
|
MDCAA |
|
58.54 |
- |
| 13 |
Lee, Michaela E (10)
|
MDGBSA |
1:00.48 |
1:05.21 |
4.73 |
| 14 |
Essa, Osob B (9)
|
MDASC |
58.91 |
1:06.15 |
7.24 |
| 15 |
Collins, MacKenzie C (10)
|
MDRAC |
1:13.03 |
1:16.21 |
3.18 |
| 16 |
Glisson, Karita (10)
|
MDGBSA |
|
1:16.38 |
- |
| 17 |
Harrison, Janiya A (10)
|
MDBCSC |
|
1:21.24 |
- |
|
Centers, Isabel A (10)
|
MDCAA |
|
NS |
- |
|
Ponton-Scott, Ashira G (9)
|
MDBCSC |
1:01.88 |
NS |
- |
|
Graham, Rilee E (10)
|
MDACA |
55.32 |
NS |
- |
|
Belgi, Anoushka A (10)
|
MDMAS |
|
NS |
- |