| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Radonovich, Rebecca N (12)
|
MDFAST |
42.32 |
40.18 |
-2.14 |
| 2 |
Bowen, Emelia J (12)
|
MDMAS |
44.57 |
41.62 |
-2.95 |
| 3 |
Correal, Aleena D (11)
|
MDMAS |
43.90 |
42.17 |
-1.73 |
| 4 |
Davalli, Addison K (11)
|
MDMAS |
43.19 |
42.23 |
-0.96 |
| 5 |
Fulton, Samantha P (12)
|
MDFAST |
47.45 |
43.62 |
-3.83 |
| 6 |
Gordy, Madeline G (12)
|
MDFAST |
45.22 |
43.83 |
-1.39 |
| 7 |
Alter, Shayna R (12)
|
MDMAS |
44.45 |
44.26 |
-0.19 |
| 8 |
Hill, Tykeisha J (11)
|
MDCSAC |
53.82 |
47.07 |
-6.75 |
| 9 |
Mahoney, Sarah C (12)
|
MDMAS |
45.69 |
47.31 |
1.62 |
| 10 |
Jones, Ryan C (12)
|
MDBCSC |
48.53 |
47.32 |
-1.21 |
| 11 |
Coates Woods, Decensae J (12)
|
MDBCSC |
50.99 |
47.68 |
-3.31 |
| 12 |
Taylor, Frances C (12)
|
MDMAS |
51.55 |
47.87 |
-3.68 |
| 13 |
Harris, Zhaniya F (12)
|
MDBCSC |
59.38 |
49.00 |
-10.38 |
| 14 |
Kefauver-Olsen, Hannah I (11)
|
MDMAS |
51.44 |
49.88 |
-1.56 |
| 15 |
Val, Brianna E (11)
|
MDMAS |
|
51.07 |
- |
| 16 |
McCarthy, Caitlin A (11)
|
MDCATY |
|
51.55 |
- |
| 17 |
Li, Yunong (12)
|
MDMAS |
51.31 |
52.82 |
1.51 |
| 18 |
DiDonato, Sophia M (11)
|
MDMAS |
|
57.07 |
- |
| 19 |
Walker, Gabrielle G (12)
|
MDMAS |
54.69 |
58.20 |
3.51 |
| 20 |
Gioffreda, Megan G (11)
|
MDMAS |
1:02.33 |
58.39 |
-3.94 |
|
Smith, Kennedy G (11)
|
MDBCSC |
1:04.75 |
DQ |
- |
|
Dalonzo, Alyssa L (12)
|
MDMAS |
|
NS |
- |