| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cooper, Addison L (10)
|
MAPAY |
41.17 |
40.24 |
-0.93 |
| 2 |
Li, Sophie (10)
|
MAKAY |
46.57 |
40.59 |
-5.98 |
| 3 |
Roulstone, Samantha J (9)
|
MAPAY |
46.99 |
43.76 |
-3.23 |
| 4 |
O'Donnell, Morgan R (10)
|
MARKRY |
44.05 |
47.01 |
2.96 |
| 5 |
Truong, Lauren (10)
|
MAPAY |
53.69 |
48.07 |
-5.62 |
| 6 |
Yuan, Carolyn Y (9)
|
MATHY |
51.15 |
48.46 |
-2.69 |
| 7 |
Flores-Caceres, Genesis G (9)
|
MAWEST |
48.52 |
50.69 |
2.17 |
| 8 |
Holland, Grace (10)
|
MAWEST |
51.72 |
51.44 |
-0.28 |
| 9 |
Coggiano, Ana M (9)
|
MDYCM |
55.81 |
53.72 |
-2.09 |
| 10 |
Lutz, Chloe A (9)
|
MAUMLY |
|
55.73 |
- |
| 11 |
Li, Belle (9)
|
MDYCM |
54.86 |
55.99 |
1.13 |
| 12 |
Matti, Abigail (10)
|
MDYCM |
57.00 |
56.64 |
-0.36 |
| 13 |
Frustaci, Olivia (9)
|
MACBFY |
56.02 |
56.93 |
0.91 |
| 14 |
Singh, Aanya (10)
|
MAPAY |
1:02.99 |
57.79 |
-5.20 |
| 15 |
Cicero, Ryann E (8)
|
MAWCAY |
55.47 |
1:00.51 |
5.04 |
| 16 |
Chen, Claire (9)
|
MAUMLY |
|
1:01.47 |
- |
| 17 |
Varani, Annalise R (9)
|
MATHY |
55.17 |
1:03.31 |
8.14 |
| 18 |
Esposito, Caitlyn A (9)
|
MDYCM |
1:00.39 |
1:04.33 |
3.94 |
| 19 |
Hirsz, Sofia (9)
|
MATHY |
1:05.00 |
1:07.78 |
2.78 |
| 20 |
Halloran, Corrine M (8)
|
MAWCAY |
1:10.00 |
1:24.54 |
14.54 |
|
Prakash, Aditi (9)
|
MAPAY |
1:10.81 |
DQ |
- |
|
Varani, Tessa P (7)
|
MATHY |
1:20.00 |
DQ |
- |