| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Whiteford, Jack D (9)
|
AMPEAQ |
41.35 |
34.94 |
-6.41 |
| 2 |
Zhao, Caden W (10)
|
AMPEAQ |
34.15 |
35.69 |
1.54 |
| 3 |
Singh, Sahil R (10)
|
AMSFAC |
36.12 |
37.20 |
1.08 |
| 4 |
Powell, Caden R (10)
|
AMBPR |
42.43 |
38.45 |
-3.98 |
| 5 |
Strock, Thomas J (10)
|
AMBPR |
37.86 |
38.76 |
0.90 |
| 6 |
Lozowski, Nicholas R (10)
|
AMSVSC |
38.76 |
38.90 |
0.14 |
| 7 |
Blocklin, Connor O (10)
|
AMBPR |
43.14 |
44.24 |
1.10 |
| 8 |
Grajeda, Santiago A (10)
|
AMWAAC |
1:06.17 |
51.35 |
-14.82 |
| 9 |
Avgerinos, Dimitrios (10)
|
AMPEAQ |
53.53 |
53.34 |
-0.19 |
|
Hagenstein, Logan D (10)
|
AMWAAC |
46.80 |
DQ |
- |
|
Sandridge, Gavin E (9)
|
AMWAAC |
1:10.00 |
DQ |
- |
|
Lewis, Liam (9)
|
AMPEAQ |
56.20 |
NS |
- |
|
Mukherjee, Josh B (10)
|
AMPEAQ |
1:05.13 |
NS |
- |