| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Powell, Caden R (10)
|
AMBPR |
42.92 |
39.09 |
-3.83 |
| 2 |
Singh, Sahil R (10)
|
AMSFAC |
43.72 |
42.67 |
-1.05 |
| 3 |
Watterson, Benjamin J (10)
|
AMMLAC |
45.77 |
45.60 |
-0.17 |
| 4 |
Blocklin, Connor O (10)
|
AMBPR |
46.34 |
45.70 |
-0.64 |
| 5 |
Borkowski, Bryce M (9)
|
AMEFSC |
48.62 |
46.97 |
-1.65 |
| 6 |
Hagenstein, Logan D (10)
|
AMWAAC |
51.60 |
47.83 |
-3.77 |
| 7 |
Guiffre, Anthony J (10)
|
AMPRA |
44.82 |
48.83 |
4.01 |
| 8 |
Hankinson, Walker (8)
|
AMUN-M |
47.34 |
48.92 |
1.58 |
| 9 |
Pelle, Tyler M (10)
|
AMEFSC |
48.19 |
49.19 |
1.00 |
| 10 |
Lange, Charles R (7)
|
AMRXA |
50.21 |
52.13 |
1.92 |
| 11 |
Pickett, Christopher B (8)
|
AMMAC |
52.05 |
52.58 |
0.53 |
|
Kadwa, Kiyan K (10)
|
AMPEAQ |
47.54 |
DQ |
- |
|
Poe, David M (9)
|
AMPEAQ |
48.08 |
NS |
- |
|
Whiteford, Jack D (10)
|
AMPEAQ |
47.30 |
NS |
- |