| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Queen, Mallory A (10)
|
VACCA |
37.14 |
37.46 |
0.32 |
| 2 |
Keister, Jacqueline T (10)
|
VAHOKI |
38.12 |
38.09 |
-0.03 |
| 3 |
Clark, Maya F (10)
|
VAHOKI |
40.27 |
40.83 |
0.56 |
| 4 |
Mackey, Jade S (10)
|
VAHOKI |
|
41.72 |
- |
| 5 |
Greer, Tesa M (10)
|
VACCA |
45.46 |
43.18 |
-2.28 |
| 6 |
Burcham, Madelyn G (10)
|
VACCA |
43.74 |
44.30 |
0.56 |
| 7 |
Sullivan, Julia M (10)
|
VACCA |
48.95 |
44.93 |
-4.02 |
| 8 |
Johnson, Teagan B (10)
|
VACCA |
49.73 |
49.24 |
-0.49 |
| 9 |
Carpenter, Lainee S (10)
|
VACCA |
46.80 |
50.42 |
3.62 |
| 10 |
Knappe, Mary G (10)
|
VACCA |
|
55.42 |
- |
| 11 |
Wikramanayake, Leila (10)
|
VACCA |
1:01.26 |
1:03.17 |
1.91 |
| 12 |
Richardson, Grace K (10)
|
VACCA |
1:08.74 |
1:05.66 |
-3.08 |