| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hughes, Jackson C (10)
|
NCWOW |
37.03 |
36.65 |
-0.38 |
| 2 |
Tillery, Wade H (10)
|
NCWOW |
43.64 |
42.89 |
-0.75 |
| 3 |
Witmer, William C (10)
|
NCWOW |
44.14 |
43.16 |
-0.98 |
| 4 |
Marlow, Tiago V (10)
|
NCECA |
44.81 |
44.78 |
-0.03 |
| 5 |
Whisnant, Mitchell W (10)
|
NCWOW |
45.68 |
45.88 |
0.20 |
| 6 |
McPherson, Connor J (9)
|
NCWOW |
48.79 |
48.64 |
-0.15 |
| 7 |
Mejouev, Maxim (10)
|
NCWOW |
49.85 |
49.17 |
-0.68 |
| 8 |
Hanson, Jack C (9)
|
NCWOW |
49.11 |
49.31 |
0.20 |
| 9 |
Wang, Maximilian (10)
|
NCWOW |
55.40 |
51.58 |
-3.82 |
| 10 |
Wert, Cooper W (9)
|
NCWOW |
|
51.84 |
- |
| 11 |
Pace, Andrew N (10)
|
NCBAC |
53.04 |
54.01 |
0.97 |
| 12 |
Reiniche, Ryan J (9)
|
NCWOW |
1:00.30 |
54.83 |
-5.47 |
| 13 |
Bollenbacher, Stephen W (9)
|
NCWOW |
|
55.68 |
- |
| 14 |
Keena, Stephen M (8)
|
NCBAC |
56.10 |
58.32 |
2.22 |
| 15 |
Williamson, Wesley C (10)
|
NCECA |
|
1:02.10 |
- |
|
Butner, Stephen A (8)
|
NCWOW |
49.58 |
DQ |
- |
|
Gnandt, Andrew J (8)
|
NCECA |
|
DQ |
- |
|
Brown, Noah D (10)
|
NCWOW |
49.88 |
NS |
- |
|
Hayes, Madden D (10)
|
NCBAC |
|
NS |
- |
|
Metty, Anderson T (10)
|
NCBAC |
46.89 |
NS |
- |