| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hughes, Jackson C (10)
|
NCWOW |
35.83 |
34.41 |
-1.42 |
| 2 |
Eickhoff, Christopher J (10)
|
NCGFSC |
37.90 |
36.75 |
-1.15 |
| 3 |
Brown, Lawson W (9)
|
NCCFAC |
37.69 |
37.77 |
0.08 |
| 4 |
Camacho, Clem C (9)
|
NCCFAC |
|
39.44 |
- |
| 5 |
Tillery, Wade H (10)
|
NCWOW |
40.92 |
39.49 |
-1.43 |
| 6 |
Clayton, William F (10)
|
NCGFSC |
45.73 |
40.72 |
-5.01 |
| 7 |
House, William E (9)
|
NCCFAC |
46.37 |
42.16 |
-4.21 |
| 8 |
Hawthorne, Maxwell S (9)
|
NCWOW |
49.21 |
45.59 |
-3.62 |
| 9 |
Hanson, Jack C (9)
|
NCWOW |
47.01 |
46.99 |
-0.02 |
| 10 |
Henry, Mason D (10)
|
NCBAC |
48.31 |
47.45 |
-0.86 |
| 11 |
Mejouev, Maxim (10)
|
NCWOW |
45.37 |
48.62 |
3.25 |
| 12 |
Reiniche, Ryan J (9)
|
NCWOW |
51.51 |
49.39 |
-2.12 |
| 13 |
Wang, Maximilian (10)
|
NCWOW |
51.31 |
49.82 |
-1.49 |
| 14 |
Wert, Cooper W (9)
|
NCWOW |
51.84 |
50.65 |
-1.19 |
| 15 |
Smith, Aiden M (10)
|
NCGFSC |
48.97 |
51.39 |
2.42 |
| 16 |
Bollenbacher, Stephen W (9)
|
NCWOW |
53.46 |
52.10 |
-1.36 |
| 17 |
Bacon, Ty M (9)
|
NCGFSC |
48.33 |
52.46 |
4.13 |
| 18 |
Hayes, Madden D (10)
|
NCBAC |
|
1:00.32 |
- |
|
Sevast, Evan S (9)
|
NCBAC |
58.20 |
DQ |
- |
|
Fiscante, Steve E (9)
|
NCCFAC |
|
NS |
- |