| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spencer, Slater W (8)
|
NCCFAC |
34.69 |
34.28 |
-0.41 |
| 2 |
Derry, Gavin M (8)
|
NCWOW |
38.87 |
41.28 |
2.41 |
| 3 |
Gu, Jiayuan (8)
|
NCWOW |
|
45.96 |
- |
| 4 |
Morris Jr., Charles A (7)
|
NCBAC |
51.56 |
48.75 |
-2.81 |
| 5 |
Yarnoz, Michael H (8)
|
NCNSEA |
51.96 |
53.30 |
1.34 |
| 6 |
Forbes, William G (8)
|
NCNSEA |
54.01 |
53.68 |
-0.33 |
| 7 |
Neyra, Carlos F (8)
|
NCCFAC |
|
55.15 |
- |
| 8 |
Keena, Patrick J (7)
|
NCBAC |
1:00.10 |
58.15 |
-1.95 |
| 9 |
Silva Gonzalez, Sabarayn (8)
|
NCCFAC |
|
1:00.17 |
- |
| 10 |
Miller, Liam S (8)
|
NCGSAT |
|
1:05.95 |
- |
| 11 |
Heckathorn, William B (8)
|
NCBAC |
|
1:10.93 |
- |
| 12 |
Henzler, Seth E (7)
|
NCCFAC |
|
1:16.54 |
- |
| 13 |
Brown, Isaiah M (7)
|
NCCFAC |
|
1:39.93 |
- |
|
Wang, Micah (6)
|
NCBAC |
1:11.25 |
NS |
- |