| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Donoghue, Rowan B (12)
|
MADST |
5:30.60 |
5:16.11 |
-14.49 |
| 2 |
Ossi, Luciana B (12)
|
PVRMSC |
5:28.48 |
5:24.90 |
-3.58 |
| 3 |
Phillips, Reagan E (12)
|
PVRMSC |
5:38.56 |
5:25.80 |
-12.76 |
| 4 |
Dworak, Andrea M (11)
|
PVRMSC |
5:33.09 |
5:30.37 |
-2.72 |
| 5 |
Traub, Celia B (12)
|
MAPAAC |
5:48.63 |
5:31.45 |
-17.18 |
| 6 |
Caponiti, Adrianna L (12)
|
PVRMSC |
5:44.88 |
5:32.25 |
-12.63 |
| 7 |
Iavarone, Siena J (12)
|
PVRMSC |
5:34.70 |
5:36.05 |
1.35 |
| 8 |
Murray, Paige C (12)
|
PVFISH |
5:39.83 |
5:38.25 |
-1.58 |
| 9 |
Crane, Zoe A (12)
|
PVRMSC |
5:49.31 |
5:38.38 |
-10.93 |
| 10 |
Mostofi, Addison B (12)
|
PVASA |
5:45.40 |
5:39.17 |
-6.23 |
| 11 |
Cheplick, Natalie A (11)
|
MAPAAC |
5:50.31 |
5:40.70 |
-9.61 |
| 12 |
Miller, Lili E (11)
|
PVRMSC |
5:54.08 |
5:45.91 |
-8.17 |
| 13 |
Schwieters, Julie K (11)
|
PVRMSC |
5:47.94 |
5:46.09 |
-1.85 |
| 14 |
Webb, Maria D (11)
|
PVRMSC |
5:53.44 |
5:47.25 |
-6.19 |
| 15 |
DeBaecke, Emerson G (11)
|
MADST |
5:57.60 |
5:49.11 |
-8.49 |
| 16 |
Cerimele, Sophia A (12)
|
MAPAAC |
5:53.00 |
5:49.75 |
-3.25 |
| 17 |
Camden, Madison N (12)
|
MAPAAC |
5:56.84 |
5:51.65 |
-5.19 |
| 18 |
Boughanem, Sabrina L (12)
|
PVFISH |
5:57.63 |
5:52.18 |
-5.45 |
| 19 |
Smith, Lauren C (11)
|
PVRMSC |
5:59.94 |
6:00.45 |
0.51 |