| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kreiley, Aidan C (14)
|
NIMERC |
2:36.18 |
2:42.36 |
6.18 |
| 2 |
Eaton, Will S (16)
|
NICLSC |
2:44.13 |
2:48.17 |
4.04 |
| 3 |
Regelsberger, Tyler R (17)
|
NIVICT |
2:49.76 |
2:51.60 |
1.84 |
| 4 |
Davis, Christian T (19)
|
NIOLSC |
2:53.93 |
2:56.85 |
2.92 |
| 5 |
Baker, Troy J (17)
|
NIVICT |
2:50.86 |
2:56.88 |
6.02 |
| 6 |
DeHollander, Erin K (12)
|
NIVICT |
3:26.72 |
3:04.49 |
-22.23 |
| 7 |
Salonen, Amber (15)
|
NISEAS |
3:06.04 |
3:04.95 |
-1.09 |
| 8 |
Hall, Harrison J (13)
|
NIVICT |
4:11.09 |
3:05.91 |
-65.18 |
| 9 |
Gasiewicz, Aaron C (12)
|
NICLSC |
3:07.07 |
3:08.35 |
1.28 |
| 10 |
Pathammavong, Suriyathai A (12)
|
NIVICT |
3:11.37 |
3:08.60 |
-2.77 |
| 11 |
Moore, Madison M (15)
|
NIVICT |
3:08.05 |
3:08.68 |
0.63 |
| 12 |
Riester, Emmaline L (16)
|
NISDSC |
3:04.08 |
3:08.72 |
4.64 |
| 13 |
Taft, Caine W (15)
|
NIMERC |
|
3:09.64 |
- |
| 14 |
Hahn, Kurt C (13)
|
NISDSC |
3:15.99 |
3:10.22 |
-5.77 |
| 15 |
Gasiewicz, Colin A (14)
|
NICLSC |
3:10.49 |
3:11.30 |
0.81 |
| 16 |
Mueller, Christopher J (14)
|
NISDSC |
3:07.66 |
3:11.70 |
4.04 |
| 17 |
Vannelli, Mitchell T (15)
|
NICLSC |
3:07.77 |
3:12.57 |
4.80 |
| 18 |
Briggs, Katharine (14)
|
NIPACK |
3:20.66 |
3:13.51 |
-7.15 |
| 19 |
Ottaviano, Catherine E (14)
|
NICSCI |
3:12.56 |
3:14.34 |
1.78 |
| 20 |
DeMartinis, Ethan W (14)
|
NIMERC |
3:30.38 |
3:14.69 |
-15.69 |
| 21 |
Senglaub, Katie (13)
|
NIVICT |
3:10.87 |
3:16.25 |
5.38 |
| 22 |
Taylor, Katie E (15)
|
NIVICT |
|
3:18.24 |
- |
| 23 |
Romanick, Lauren A (15)
|
NIPACK |
3:18.69 |
3:19.23 |
0.54 |
| 24 |
Warner, Tyler J (13)
|
NIMETS |
3:31.88 |
3:23.96 |
-7.92 |
| 25 |
Gartner, Sarah N (16)
|
NIGCOM |
3:19.81 |
3:24.46 |
4.65 |
| 26 |
Davison, Asella M (14)
|
NISDSC |
3:51.39 |
3:54.28 |
2.89 |
| 27 |
Langlotz, Olivia M (13)
|
NICLSC |
4:14.37 |
4:02.30 |
-12.07 |
|
Ludlow, Allison G (18)
|
NIMERC |
3:12.59 |
DQ |
- |