| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Esposito, Grace A (14)
|
MRFREE |
26.30 |
26.26 |
-0.04 |
| 2 |
Huang, Cheri K (14)
|
MRFREE |
26.19 |
26.41 |
0.22 |
| 3 |
Chin, Cassidy K (13)
|
MRFREE |
27.08 |
27.08 |
0.00 |
| 4 |
Zhang, Suri (13)
|
MRFREE |
27.57 |
27.09 |
-0.48 |
| 5 |
Triolo, Alexia S (14)
|
MRFREE |
28.42 |
28.20 |
-0.22 |
| 6 |
Lally, Keely M (14)
|
MRFREE |
27.52 |
28.29 |
0.77 |
| 7 |
Roudnitsky, Julia (13)
|
MRFREE |
29.44 |
28.82 |
-0.62 |
| 8 |
Shnayderman, Alisa (13)
|
MRMPNY |
29.17 |
28.83 |
-0.34 |
| 9 |
Perpignan, Marleigh A (13)
|
MRFREE |
30.41 |
29.17 |
-1.24 |
| 10 |
Sheehan, Maeve E (14)
|
MRFREE |
29.90 |
29.28 |
-0.62 |
| 11 |
Reid, Morgan N (13)
|
MRFREE |
29.70 |
29.74 |
0.04 |
| 12 |
Gallo, Emma J (14)
|
MRFREE |
30.99 |
30.85 |
-0.14 |
| 13 |
Mitchell, Grace A (14)
|
MRFREE |
30.95 |
30.88 |
-0.07 |
| 14 |
Gagliardotto, Giada V (13)
|
MRFREE |
33.50 |
33.80 |
0.30 |