| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bronskikh, Valeriia (13)
|
MRUNSH |
|
26.85 |
- |
| 2 |
Jia, Nicole X (13)
|
MRMPNY |
26.79 |
27.81 |
1.02 |
| 3 |
Shnayderman, Alisa (13)
|
MRMPNY |
27.15 |
27.91 |
0.76 |
| 4 |
Epps, Emani N (13)
|
MRBKR |
27.86 |
28.11 |
0.25 |
| 5 |
Cohen, Alexis M (14)
|
MRBKR |
28.28 |
28.33 |
0.05 |
| 6 |
Troncoso, Emma H (13)
|
MRFREE |
31.78 |
29.81 |
-1.97 |
| 7 |
Ayupova, Maya (13)
|
MRSHY |
35.08 |
31.47 |
-3.61 |
| 8 |
Sanchez, Valentina S (13)
|
MRFREE |
31.61 |
31.49 |
-0.12 |
| 9 |
Ellison, Cori C (13)
|
MRBKR |
31.56 |
31.84 |
0.28 |
| 10 |
Nathanson, Michelle (14)
|
MRFREE |
33.26 |
32.24 |
-1.02 |
| 11 |
Leon Rosenfield, Carine D (13)
|
MRBKR |
33.31 |
32.61 |
-0.70 |
| 12 |
McGee, Leah T (13)
|
MRBKR |
32.10 |
32.79 |
0.69 |
| 13 |
Smith, Gabrielle R (13)
|
MRBKR |
33.74 |
32.89 |
-0.85 |
| 14 |
Irizarry, Chloe C (13)
|
MRBKR |
33.31 |
33.00 |
-0.31 |
| 15 |
Titievsky, Zoe F (13)
|
MRBKR |
35.23 |
33.35 |
-1.88 |
| 16 |
Hall, Emma B (13)
|
MRBKR |
34.36 |
33.75 |
-0.61 |
| 17 |
Seymour, Nayelly (13)
|
NJWWAT |
31.55 |
34.89 |
3.34 |
| 18 |
Albert, Victoria E (13)
|
MRBKR |
37.27 |
36.12 |
-1.15 |
| 19 |
Torres, Amaliah J (13)
|
MRFREE |
37.09 |
36.63 |
-0.46 |
| 20 |
Gelashvili, Elizaveta L (13)
|
MRMPNY |
40.26 |
39.10 |
-1.16 |