| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reilly, Bridget F (10)
|
NISTAR |
3:24.53 |
3:28.13 |
3.60 |
| 2 |
Choroser, Penelope T (10)
|
NITWST |
3:36.44 |
3:33.56 |
-2.88 |
| 3 |
Murphy, Campbell E (10)
|
NITTSC |
|
3:42.80 |
- |
| 4 |
Burnham, Madeline (9)
|
NIVELO |
3:41.26 |
3:46.31 |
5.05 |
| 5 |
Loehfelm, Haley E (10)
|
NITWST |
3:48.79 |
3:46.33 |
-2.46 |
| 6 |
Amer, Sarah F (9)
|
NIVELO |
3:51.99 |
3:50.64 |
-1.35 |
| 7 |
Umansky, Katarina R (8)
|
NIBAAC |
|
4:07.30 |
- |
| 8 |
Alessi, Luciana M (9)
|
NIBAAC |
|
4:12.53 |
- |
| 9 |
Bogunovic, Leonora (9)
|
NIVELO |
4:05.86 |
4:13.61 |
7.75 |
| 10 |
Hutton, Ryan (9)
|
NIVELO |
4:16.80 |
4:13.71 |
-3.09 |
| 11 |
Tothero, Madeleine S (10)
|
NISTAR |
4:03.11 |
4:14.73 |
11.62 |
| 12 |
Wands, Lucille M (10)
|
NIVELO |
4:14.92 |
4:20.12 |
5.20 |
| 13 |
Adams, Mallory B (8)
|
NIBAAC |
|
4:33.63 |
- |
|
Washington, Ashanti M (9)
|
NISTAR |
|
DQ |
- |
|
Ryan, Isabella A (9)
|
NIVELO |
|
DQ |
- |