| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hetrick, Paige M (16)
|
NIUN-N |
2:35.26 |
2:29.84 |
-5.42 |
| 2 |
Hawkins, Emma (14)
|
NIVICT |
2:29.88 |
2:32.64 |
2.76 |
| 3 |
Regelsberger, Emily C (15)
|
NIVICT |
2:47.01 |
2:34.44 |
-12.57 |
| 4 |
Royce-Roll, Willa H (15)
|
NIKRKN |
2:29.65 |
2:34.79 |
5.14 |
| 5 |
Killion, Natalie A (13)
|
NICLSC |
2:37.97 |
2:35.91 |
-2.06 |
| 6 |
McDonnell, Lillian (17)
|
NISTAR |
2:35.60 |
2:36.92 |
1.32 |
| 7 |
Conley, Sarah L (16)
|
NITWST |
2:32.95 |
2:37.07 |
4.12 |
| 8 |
Di Persio, Mara (17)
|
BROC |
2:30.79 |
2:38.27 |
7.48 |
| 9 |
Ritz, Erin (16)
|
NIVICT |
2:37.54 |
2:38.62 |
1.08 |
| 10 |
Senglaub, Katie (14)
|
NIVICT |
2:36.21 |
2:40.37 |
4.16 |
| 11 |
Webster, Laryssa G (13)
|
NIUN-N |
2:34.46 |
2:45.74 |
11.28 |
| 12 |
Van Vreede, Olivia L (14)
|
NIVICT |
|
2:46.21 |
- |
| 13 |
Lindberg, Gwendolyn A (15)
|
NIKRKN |
|
2:47.06 |
- |
| 14 |
Gardner, Morgan M (17)
|
NISTAR |
2:52.48 |
2:48.21 |
-4.27 |
| 15 |
Pathammavong, Suriyathai A (13)
|
NIVICT |
2:53.05 |
2:50.21 |
-2.84 |
| 16 |
Moore, Madison M (15)
|
NIVICT |
2:38.30 |
2:52.02 |
13.72 |
| 17 |
Blake, Emily C (14)
|
NISTAR |
2:39.17 |
2:52.21 |
13.04 |
| 18 |
Senglaub, Emma (14)
|
NIVICT |
2:48.20 |
2:52.63 |
4.43 |
| 19 |
Sherlock, Jenna G (16)
|
NISTAR |
2:55.67 |
2:53.00 |
-2.67 |
| 20 |
Holland, Elle C (17)
|
NITWST |
2:55.21 |
2:54.73 |
-0.48 |
| 21 |
Lee, Madeline E (14)
|
NIVELO |
2:54.20 |
2:57.69 |
3.49 |
| 22 |
Tesluk, Helen E (14)
|
NISTAR |
2:58.81 |
2:58.36 |
-0.45 |
| 23 |
DeHollander, Erin K (13)
|
NIVICT |
|
3:04.20 |
- |
| 24 |
Jefferis, Ilah M (13)
|
NIKRKN |
|
3:04.86 |
- |
| 25 |
Hesch, Natalie G (14)
|
NITTSC |
3:50.02 |
3:14.60 |
-35.42 |
| 26 |
Faust, Ariel (16)
|
NIVELO |
|
3:16.91 |
- |
| 27 |
Gentile, Juliana (14)
|
NIVELO |
3:17.64 |
3:18.47 |
0.83 |
| 28 |
Hall, Sofia F (13)
|
NIBAAC |
3:17.71 |
3:18.62 |
0.91 |
| 29 |
Hull, Emily G (13)
|
NISTAR |
|
3:23.59 |
- |
|
Mueller, Maegan G (14)
|
NIVELO |
|
DNF |
- |