| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hodge, Ashley S (12)
|
NIUN-D |
2:42.52 |
2:42.77 |
0.25 |
| 2 |
Wu, Helen (12)
|
NIUN-D |
2:52.00 |
2:50.15 |
-1.85 |
| 3 |
Pathammavong, Suriyathai A (12)
|
NIUN-V |
2:51.16 |
2:51.59 |
0.43 |
| 4 |
Levy, Lauren C (11)
|
NITTSC |
|
2:57.19 |
- |
| 5 |
Killion, Natalie A (11)
|
NICLSC |
|
2:57.94 |
- |
| 6 |
Donnelly, Keira (12)
|
NIVICT |
3:14.56 |
3:01.45 |
-13.11 |
| 7 |
Sandruck, Brynn A (12)
|
NIVICT |
2:58.01 |
3:07.89 |
9.88 |
| 8 |
Moore, Paige E (12)
|
NIUN-D |
3:15.49 |
3:08.58 |
-6.91 |
| 9 |
Pirigyi, Sophia J (11)
|
NISTAR |
|
3:15.65 |
- |
| 10 |
Chaplin, Allison V (12)
|
NITTSC |
|
3:24.52 |
- |
| 11 |
Mattson, Catherine C (11)
|
NICLSC |
3:26.86 |
3:24.69 |
-2.17 |
| 12 |
Hauser-Howells, Lillian K (12)
|
NITTSC |
|
3:35.62 |
- |