| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kabunov, Mikhail V (12)
|
NIUN |
26.42 |
26.54 |
0.12 |
| 2 |
Van Kersbergen, Gavin M (11)
|
NIUN |
27.50 |
27.15 |
-0.35 |
| 3 |
Bond, Shane P (11)
|
NIOLSC |
29.62 |
29.70 |
0.08 |
| 4 |
VanAlst, Amon J (12)
|
ADUN-A |
31.09 |
30.69 |
-0.40 |
| 5 |
Carbonaro, Charlie P (11)
|
NIAYST |
30.85 |
31.85 |
1.00 |
| 6 |
Tarolli, Angus J (12)
|
NICSCI |
|
32.21 |
- |
| 7 |
Milliman, Kaden M (12)
|
NIWGSC |
33.12 |
32.30 |
-0.82 |
| 8 |
Zens, Lucien M (12)
|
NICSCI |
33.51 |
32.97 |
-0.54 |
| 9 |
Pelin, Tyler H (12)
|
NINAYE |
35.84 |
36.51 |
0.67 |
| 10 |
Tsymbala, Denys (12)
|
NICSCI |
41.91 |
36.81 |
-5.10 |
| 11 |
Keiling, Herman M (12)
|
NICSCI |
|
36.88 |
- |
| 12 |
MacLachlan, Nathan W (12)
|
NICSCI |
55.00 |
38.03 |
-16.97 |
| 13 |
Limpert, Ryan P (11)
|
NICSCI |
37.33 |
38.07 |
0.74 |
| 14 |
Mathews, Benjamin A (12)
|
NICSCI |
|
39.13 |
- |
| 15 |
Purdy, Aaron C (12)
|
NINAYE |
1:10.00 |
43.05 |
-26.95 |