| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fernandez-Turo, Rocio A (12)
|
ADNHA |
41.93 |
41.17 |
-0.76 |
| 2 |
Elmarasy, Noor M (12)
|
ADNHA |
1:00.29 |
44.03 |
-16.26 |
| 3 |
Heiner, Elizabeth A (12)
|
ADNHA |
44.88 |
44.47 |
-0.41 |
| 4 |
Kelly, Teagan M (11)
|
ADCANA |
44.22 |
45.39 |
1.17 |
| 5 |
Trafton, Abbie C (11)
|
ADBENN |
45.96 |
45.70 |
-0.26 |
| 6 |
Jenkins, Clara C (11)
|
ADNHA |
52.16 |
46.07 |
-6.09 |
| 7 |
Applebaum, Aaliyah R (12)
|
ADCANA |
44.77 |
46.40 |
1.63 |
| 8 |
Huettner, Alexa R (11)
|
ADSCHE |
46.85 |
50.04 |
3.19 |
| 9 |
Alcuri, Julia M (12)
|
ADNHA |
52.59 |
50.05 |
-2.54 |
| 10 |
Luley, Mya E (12)
|
ADNHA |
50.07 |
51.42 |
1.35 |
| 11 |
Gioppo, Abigail M (12)
|
ADNHA |
51.26 |
52.74 |
1.48 |
| 12 |
Dugan, Chrisgianna R (12)
|
ADSCHE |
1:01.71 |
54.14 |
-7.57 |
| 13 |
Patel, Avni B (11)
|
ADCLRK |
55.36 |
57.60 |
2.24 |
| 14 |
Cerruto, Valerie L (11)
|
ADCANA |
53.17 |
58.59 |
5.42 |
| 15 |
Bong, Laila G (11)
|
ADCLRK |
1:08.11 |
1:06.63 |
-1.48 |
|
Budhu, Sonia M (12)
|
ADNHA |
|
DQ |
- |
|
Lebert, Hannah M (11)
|
ADBENN |
47.67 |
DQ |
- |
|
Corey, Madeline C (12)
|
ADSCHE |
|
NS |
- |
|
Coulombe, Zoey I (11)
|
ADSCHE |
47.12 |
NS |
- |