| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
French, Matthew M (12)
|
ADNHA |
|
45.03 |
- |
| 2 |
Tieckelmann, Connor R (11)
|
ADSCHE |
43.87 |
45.65 |
1.78 |
| 3 |
Hubbard, Ethan A (12)
|
ADCANA |
46.95 |
46.65 |
-0.30 |
| 4 |
Walsh, Andrew R (12)
|
ADSCHE |
45.44 |
46.74 |
1.30 |
| 5 |
Murphy, James C (11)
|
ADBENN |
53.66 |
47.20 |
-6.46 |
| 6 |
Splonskowski, Brendan J (11)
|
ADJJSC |
46.69 |
47.43 |
0.74 |
| 7 |
Wang, Jake H (11)
|
ADSCHE |
54.91 |
52.99 |
-1.92 |
| 8 |
D'Amico, David A (11)
|
ADSCHE |
51.24 |
54.08 |
2.84 |
| 9 |
Robinson, Samuel J (11)
|
ADCANA |
55.13 |
57.23 |
2.10 |
|
Barlow, Nicholas M (11)
|
ADCANA |
52.17 |
DQ |
- |
|
Stabinski, Alexander J (11)
|
ADCANA |
|
DQ |
- |
|
Dhillon, Meherpratap S (12)
|
ADADDI |
48.61 |
NS |
- |
|
White, Sebastian A (11)
|
ADBENN |
56.51 |
NS |
- |
|
Hanna, Nathan C (11)
|
ADCLIN |
42.94 |
NS |
- |
|
Scerbo, Caleb F (11)
|
ADCLIN |
47.69 |
NS |
- |
|
Sprouse, Jack E (12)
|
ADSVP |
1:03.52 |
NS |
- |