| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zuo, Bonita R (12)
|
MAUN-0 |
33.83 |
32.89 |
-0.94 |
| 1 |
Yao, Juliana L (11)
|
MAMSA |
41.73 |
35.72 |
-6.01 |
| 1 |
Fickett, Emma R (12)
|
MAHAC |
40.02 |
37.79 |
-2.23 |
| 2 |
Ying, Joy (12)
|
MAMSA |
36.63 |
35.02 |
-1.61 |
| 2 |
Buehler, Katie H (12)
|
MAKA |
45.41 |
35.79 |
-9.62 |
| 2 |
Buck, Ava S (12)
|
MAHAC |
38.66 |
37.98 |
-0.68 |
| 3 |
Kil, Samantha A (12)
|
MAKA |
37.46 |
35.32 |
-2.14 |
| 3 |
Bair, Camryn N (12)
|
MANLAC |
|
36.22 |
- |
| 4 |
Scime, Alex A (11)
|
MAGRA |
36.65 |
35.66 |
-0.99 |
| 4 |
Mulroy, Montana N (12)
|
MAEPAC |
|
39.17 |
- |
| 5 |
Leonard, Allison J (12)
|
MAHAC |
44.22 |
39.98 |
-4.24 |
| 6 |
Schultz, Gabrielle S (12)
|
MAFWAC |
|
40.50 |
- |
| 7 |
Gerlinski, Emma K (12)
|
MAGSVY |
42.60 |
40.60 |
-2.00 |
| 8 |
Rasmussen, Charlotte B (11)
|
MAFWAC |
42.02 |
40.68 |
-1.34 |
| 9 |
Bishop, Lucille E (12)
|
MAMSC |
42.70 |
40.97 |
-1.73 |
| 10 |
Wilkinson, Isabella J (12)
|
MAUN-0 |
|
41.50 |
- |
| 11 |
Rockwell, Suha S (11)
|
MAEPAC |
|
41.57 |
- |
| 12 |
Koratich, Kylee S (12)
|
MAUN-0 |
|
41.87 |
- |
| 13 |
Tallapragada, Harshitha T (11)
|
MAMSAU |
43.50 |
42.32 |
-1.18 |
| 14 |
Phillips, Gabrielle S (11)
|
MAMSA |
45.94 |
43.18 |
-2.76 |
| 15 |
Rafeld, Ella J (11)
|
MAPSC |
|
44.12 |
- |
| 16 |
Turner, Hannah M (11)
|
MAEPAC |
47.72 |
44.63 |
-3.09 |
| 17 |
Taylor, Madeline C (12)
|
MAMSC |
|
44.69 |
- |
| 18 |
Miller, Claire B (11)
|
MAFSSC |
55.79 |
46.64 |
-9.15 |
| 19 |
Meckley, Jordan M (12)
|
MAUN-0 |
45.13 |
46.96 |
1.83 |
| 20 |
Hestnar, Meredith R (11)
|
MAUDAC |
|
47.03 |
- |
| 21 |
Primas, Grace L (12)
|
MAEPAC |
52.12 |
47.09 |
-5.03 |
| 22 |
East, Margaret A (12)
|
MAMSA |
|
58.82 |
- |
|
Hasis, Nicole K (11)
|
MAKAUN |
40.37 |
38.01 |
-2.36 |
|
Woronko, Julia M (11)
|
MAHAC |
37.90 |
NS |
- |