| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blough, Madelyn G (10)
|
MAKA |
42.06 |
40.76 |
-1.30 |
| 1 |
Persun, Kiley N (10)
|
MAWASC |
53.04 |
50.18 |
-2.86 |
| 2 |
Wigginton, Samantha L (10)
|
MAHHAA |
43.22 |
42.96 |
-0.26 |
| 2 |
Summer, Megan E (9)
|
MAHHAA |
48.67 |
50.19 |
1.52 |
| 3 |
Gordon, Ava E (10)
|
MDFAST |
45.19 |
45.48 |
0.29 |
| 3 |
Ivall, Olivia P (10)
|
MDFAST |
55.51 |
52.61 |
-2.90 |
| 4 |
Mothersbaugh, Eloise F (10)
|
MAHHAA |
47.52 |
46.72 |
-0.80 |
| 4 |
Brashear, Johanna H (10)
|
MDFAST |
56.38 |
52.91 |
-3.47 |
| 5 |
Rawa, Ashley E (10)
|
MAHHAA |
|
53.24 |
- |
| 6 |
Pieklo, Evalyn M (10)
|
MDFAST |
54.90 |
57.11 |
2.21 |
| 7 |
Nelson, Marleigh A (10)
|
MDFAST |
|
57.52 |
- |
|
O'Connor, Bryn C (10)
|
MAHHAA |
45.64 |
43.81 |
-1.83 |
|
Jones, Camryn L (10)
|
MAHHAA |
|
45.96 |
- |