| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Qiu, Janice S (11)
|
NCTAC |
45.59 |
44.86 |
-0.73 |
| 2 |
Hauser, Adrienne G (12)
|
NCLTNC |
51.46 |
45.63 |
-5.83 |
| 3 |
Komelasky, Sydney M (11)
|
NCCKS |
50.56 |
45.85 |
-4.71 |
| 4 |
O'Leary, Marissa J (12)
|
NCLTNC |
48.21 |
47.23 |
-0.98 |
| 5 |
Kalikiri, Thannishtha Reddy (12)
|
NCTAC |
|
47.41 |
- |
| 6 |
Hobbs, Haeley M (12)
|
NCTAC |
48.45 |
47.95 |
-0.50 |
| 7 |
Nakada, Riri (12)
|
NCTAC |
|
48.06 |
- |
| 8 |
Krutova, Zlata (11)
|
NCTAC |
47.66 |
48.14 |
0.48 |
| 9 |
Zoller, Eleanor M (11)
|
NCTAC |
51.92 |
51.25 |
-0.67 |
| 10 |
Hammer, Naomi R (11)
|
NCTAC |
|
54.61 |
- |
| 11 |
Cherukuri, Sahrudee S (11)
|
NCLTNC |
1:03.17 |
54.84 |
-8.33 |
|
Koneru, Dhriti N (12)
|
NCTAC |
56.26 |
DQ |
- |
|
Chakkravarthy, Cynthia B (11)
|
NCTAC |
1:24.90 |
DQ |
- |
|
Campos Fiorati, Camila (11)
|
NCTAC |
55.11 |
DQ |
- |
|
Zheng, Claire N (11)
|
NCTAC |
1:06.20 |
DQ |
- |
|
Evans, Sophie L (11)
|
NCTAC |
49.14 |
NS |
- |
|
Smith, Alexa C (11)
|
NCTAC |
55.67 |
NS |
- |