| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moran, Neve A (12)
|
MDCAA |
2:55.02 |
2:54.99 |
-0.03 |
| 2 |
von Wachter, Elizabeth C (11)
|
MDEST |
2:58.18 |
2:55.07 |
-3.11 |
| 3 |
Ketler, Ellie R (12)
|
MDCAA |
2:55.19 |
2:57.41 |
2.22 |
| 4 |
Chung, Julianne S (12)
|
MDCAA |
2:56.52 |
3:02.33 |
5.81 |
| 5 |
Wilson, Braelynn G (11)
|
MDGBSA |
3:05.72 |
3:02.51 |
-3.21 |
| 6 |
Grey, Emily L (12)
|
MDASC |
3:17.84 |
3:03.17 |
-14.67 |
| 7 |
Stage, Amari S (12)
|
MDACA |
3:01.84 |
3:04.21 |
2.37 |
| 8 |
Stuhlmacher, Kate A (12)
|
MDCAA |
3:06.83 |
3:04.83 |
-2.00 |
| 9 |
Pannell, Addison E (12)
|
MDFAST |
3:01.97 |
3:05.00 |
3.03 |
| 10 |
Birdsall, Kathleen S (12)
|
MDEST |
3:09.76 |
3:07.26 |
-2.50 |
| 11 |
Morrison, Greer E (12)
|
MDASC |
3:09.22 |
3:07.56 |
-1.66 |
| 12 |
Matkovic, Sophia I (12)
|
MDCAA |
3:10.02 |
3:09.08 |
-0.94 |
| 13 |
Sorenson, Audrey E (12)
|
MDRAC |
3:06.93 |
3:10.49 |
3.56 |
| 14 |
Daley, Susanna M (12)
|
MDMAS |
3:13.44 |
3:19.38 |
5.94 |