| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaines, Laila K (10)
|
PVPM |
32.31 |
31.46 |
-0.85 |
| 2 |
Beck, Elizabeth L (10)
|
PVPM |
34.46 |
34.54 |
0.08 |
| 3 |
Long, Charlotte G (10)
|
PVPM |
37.51 |
37.99 |
0.48 |
| 4 |
Gines, Victoria L (9)
|
PVPM |
38.72 |
38.77 |
0.05 |
| 5 |
Mancari, Emerson K (9)
|
PVPM |
41.21 |
39.52 |
-1.69 |
| 6 |
Weingart, Mara J (10)
|
PVPM |
42.11 |
39.58 |
-2.53 |
| 7 |
Powell-Switaj, Zoe R (9)
|
PVPM |
42.75 |
40.19 |
-2.56 |
| 8 |
Montilla, Jocelyn A (10)
|
PVPM |
37.62 |
41.60 |
3.98 |
| 9 |
Andress, Charlotte M (10)
|
PVPM |
|
41.74 |
- |
| 10 |
Hank, Jamie W (10)
|
PVPM |
46.30 |
42.01 |
-4.29 |
| 11 |
Maldarelli, Emma K (10)
|
PVPM |
|
43.08 |
- |
| 12 |
Evans, Melinda E (9)
|
PVPM |
44.04 |
44.03 |
-0.01 |
| 13 |
Rice, Lily D (9)
|
PVPM |
47.24 |
44.23 |
-3.01 |
| 14 |
Alessi, Corinne E (9)
|
PVPM |
46.17 |
45.53 |
-0.64 |
| 15 |
Wall, Emma N (9)
|
PVPM |
|
53.48 |
- |
|
Don, Audrey H (9)
|
PVPM |
49.25 |
NS |
- |
|
Stark Puglisi, Chloe G (9)
|
PVPM |
41.11 |
NS |
- |