| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chen, Guanyan D (10)
|
NCNCAC |
36.76 |
35.17 |
-1.59 |
| 2 |
Collins, Daisy B (10)
|
NCNCAC |
34.17 |
35.28 |
1.11 |
| 3 |
Brock, Yari E (9)
|
NCNCAC |
39.35 |
37.82 |
-1.53 |
| 4 |
Biswas, Emma A (10)
|
NCNCAC |
38.31 |
38.85 |
0.54 |
| 5 |
Turner, Elle G (9)
|
NCRSA |
40.94 |
39.42 |
-1.52 |
| 6 |
Werley, Ella D (10)
|
NCNCAC |
44.90 |
39.61 |
-5.29 |
| 7 |
Locklear, Marissa N (10)
|
NCNCAC |
39.44 |
39.80 |
0.36 |
| 8 |
Honnold, Emory C (10)
|
NCRSA |
38.89 |
41.41 |
2.52 |
| 9 |
Romano, Dakota M (10)
|
NCRSA |
40.60 |
43.65 |
3.05 |
| 10 |
Khandani, Hannah R (9)
|
NCNCAC |
42.35 |
44.58 |
2.23 |
| 11 |
DeLoreto, Dorothea A (9)
|
NCSGSA |
42.92 |
44.66 |
1.74 |
| 12 |
Monroe, Mia E (10)
|
NCNCAC |
39.94 |
45.08 |
5.14 |
| 13 |
Meiferdt, Annalie S (8)
|
UNAT |
46.68 |
47.00 |
0.32 |
| 14 |
McFeaters, Brooklyn A (9)
|
NCRSA |
50.42 |
48.54 |
-1.88 |
| 15 |
Keelor, Claire E (9)
|
NCRSA |
|
52.99 |
- |
| 15 |
Espinosa, Madisyn G (9)
|
NCSGSA |
54.47 |
52.99 |
-1.48 |
| 17 |
Jacobs, Sophie G (8)
|
NCNCAC |
|
53.44 |
- |
| 18 |
Overstreet, Ainsley C (9)
|
NCNCAC |
1:00.00 |
56.21 |
-3.79 |
| 19 |
Silvernail, Meghan L (10)
|
NCSSS |
58.56 |
57.40 |
-1.16 |
| 20 |
Finucane, Elinor P (9)
|
NCRSA |
55.15 |
57.84 |
2.69 |
| 21 |
Donovan, Eleanor G (8)
|
NCRSA |
1:00.99 |
58.59 |
-2.40 |
| 22 |
Drexel, Isabelle M (10)
|
NCSGSA |
44.66 |
59.80 |
15.14 |
| 23 |
Haithcock, Caroline (9)
|
NCRSA |
1:04.88 |
1:01.80 |
-3.08 |
| 24 |
Delgado, Madison F (10)
|
NCNCAC |
|
1:02.26 |
- |
| 25 |
Kraft, Ellie H (10)
|
NCRSA |
|
1:02.50 |
- |
| 26 |
Raghunathan, Aarya V (10)
|
NCNCAC |
|
1:03.12 |
- |
| 27 |
Catalano, Isaelle L (10)
|
NCRSA |
48.99 |
1:04.79 |
15.80 |
|
James, Peyton A (9)
|
NCNCAC |
1:00.00 |
DQ |
- |
|
Bomberger, Claire D (9)
|
NCRSA |
|
NS |
- |