| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wolk, Isabel H (12)
|
NCNCAC |
29.70 |
28.96 |
-0.74 |
| 2 |
Emrich, Abigail G (12)
|
NCNCAC |
28.89 |
28.99 |
0.10 |
| 3 |
Shuping, Avery E (12)
|
NCNCAC |
31.62 |
30.93 |
-0.69 |
| 4 |
Collins, Mackenzie K (11)
|
NCNCAC |
32.62 |
32.11 |
-0.51 |
| 5 |
Chen, Ruoyan D (12)
|
NCNCAC |
36.01 |
32.69 |
-3.32 |
| 6 |
Giovanello, Aurora J (12)
|
NCNCAC |
35.41 |
35.21 |
-0.20 |
| 7 |
Kim, Kenzie A (11)
|
NCNCAC |
36.34 |
36.38 |
0.04 |
| 8 |
Thomas, Arianna M (11)
|
NCNCAC |
36.25 |
37.29 |
1.04 |
| 9 |
Burton, Chloe G (11)
|
NCNCAC |
38.23 |
38.34 |
0.11 |
| 10 |
Dever, Emerson B (12)
|
NCNCAC |
47.35 |
38.73 |
-8.62 |
| 11 |
Taub, Anna J (12)
|
NCNCAC |
39.43 |
39.46 |
0.03 |
| 12 |
Milsom, Molly M (12)
|
NCNCAC |
40.81 |
40.70 |
-0.11 |
| 13 |
Li, Joie N (11)
|
NCNCAC |
41.65 |
41.64 |
-0.01 |
| 14 |
Yao, Cana (11)
|
NCNCAC |
44.82 |
44.36 |
-0.46 |
| 15 |
Li, Angela (11)
|
NCNCAC |
52.89 |
47.30 |
-5.59 |
| 16 |
Lee, Isabelle A (11)
|
NCNCAC |
55.99 |
48.48 |
-7.51 |
|
Brav, Sapir (12)
|
NCNCAC |
1:00.99 |
DQ |
- |