| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wolk, Isabel H (12)
|
NCNCAC |
26.19 |
25.54 |
-0.65 |
| 2 |
Emrich, Abigail G (12)
|
NCNCAC |
27.99 |
27.44 |
-0.55 |
| 3 |
Shuping, Avery E (12)
|
NCNCAC |
28.15 |
27.74 |
-0.41 |
| 4 |
Collins, Mackenzie K (11)
|
NCNCAC |
29.07 |
28.44 |
-0.63 |
| 5 |
Giovanello, Aurora J (12)
|
NCNCAC |
29.99 |
29.61 |
-0.38 |
| 6 |
Thomas, Arianna M (11)
|
NCNCAC |
30.90 |
30.92 |
0.02 |
| 7 |
Kim, Kenzie A (11)
|
NCNCAC |
32.04 |
31.20 |
-0.84 |
| 8 |
Dever, Emerson B (12)
|
NCNCAC |
34.04 |
31.76 |
-2.28 |
| 9 |
Taub, Anna J (12)
|
NCNCAC |
34.17 |
32.03 |
-2.14 |
| 10 |
Burton, Chloe G (11)
|
NCNCAC |
35.95 |
32.06 |
-3.89 |
| 11 |
Milsom, Molly M (12)
|
NCNCAC |
35.64 |
34.42 |
-1.22 |
| 12 |
Yao, Cana (11)
|
NCNCAC |
39.73 |
36.38 |
-3.35 |
| 13 |
Li, Joie N (11)
|
NCNCAC |
38.24 |
36.48 |
-1.76 |
| 14 |
Li, Angela (11)
|
NCNCAC |
39.63 |
37.68 |
-1.95 |
| 15 |
Lee, Isabelle A (11)
|
NCNCAC |
45.99 |
38.82 |
-7.17 |
| 16 |
Brav, Sapir (12)
|
NCNCAC |
41.78 |
39.81 |
-1.97 |
| 17 |
Wang, Rui (11)
|
NCNCAC |
50.99 |
45.61 |
-5.38 |