| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Boehlke, Eva V (13)
|
NCNCAC |
5:30.90 |
5:26.66 |
-4.24 |
| 2 |
Cinque, Karissa L (13)
|
NCNCAC |
5:38.62 |
5:37.37 |
-1.25 |
| 3 |
Sciaudone, Rose M (13)
|
NCMOR |
5:42.17 |
5:39.83 |
-2.34 |
| 4 |
Dungan, Kristin L (13)
|
NCYOTA |
5:38.87 |
5:43.54 |
4.67 |
| 5 |
Kozubowski, Caroline C (13)
|
NCMOR |
5:44.14 |
5:43.99 |
-0.15 |
| 6 |
Georgallis, Sofia C (13)
|
NCNCAC |
6:04.49 |
5:51.16 |
-13.33 |
| 7 |
Pegram, Elizabeth C (13)
|
NCRSA |
6:00.98 |
6:00.79 |
-0.19 |
| 8 |
Ahart, Savannah K (14)
|
NCRSA |
6:12.51 |
6:01.28 |
-11.23 |
| 9 |
Petersen, Riley C (13)
|
NCWAVE |
6:06.62 |
6:13.42 |
6.80 |
| 10 |
Kilgallen, Emily K (13)
|
NCYOTA |
6:23.91 |
6:14.50 |
-9.41 |
| 11 |
Grossmann, Willow (14)
|
NCMOR |
6:11.89 |
6:15.58 |
3.69 |
| 12 |
Caulway, Allison J (13)
|
NCYOTA |
6:27.00 |
6:19.02 |
-7.98 |
| 13 |
Aparicio, Analiyah I (14)
|
NCRSA |
6:43.96 |
6:25.31 |
-18.65 |
| 14 |
Neudecker, Layne E (13)
|
NCYOTA |
6:45.36 |
6:54.49 |
9.13 |