| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Davis, Lili G (7)
|
NCQCD |
21.10 |
21.04 |
-0.06 |
| 2 |
Millsaps, Katherine G (6)
|
NCCVAC |
21.92 |
22.83 |
0.91 |
| 3 |
Stancil, Ellie G (8)
|
NCGG |
24.03 |
23.17 |
-0.86 |
| 4 |
Cable, Meris A (8)
|
NCGG |
25.37 |
23.58 |
-1.79 |
| 5 |
Ramsey, Chloe L (8)
|
NCWA |
|
25.36 |
- |
| 6 |
Bartlett, Veronica D (7)
|
NCCVAC |
28.51 |
26.45 |
-2.06 |
| 7 |
King, Maigue C (8)
|
NCATOM |
|
29.18 |
- |
| 8 |
Young, Makayla O (8)
|
NCQCD |
27.33 |
29.25 |
1.92 |
| 9 |
Sellars, Riley L (8)
|
NCQCD |
28.17 |
29.93 |
1.76 |
| 10 |
Taylor, Martha K (8)
|
NCATOM |
|
30.14 |
- |
| 11 |
Beltran-Trejo, Fernanda (7)
|
NCCVAC |
31.02 |
31.02 |
0.00 |
|
Millsaps, Caroline M (5)
|
NCCVAC |
|
DQ |
- |
|
Wilson, Riley E (5)
|
NCWST |
|
DQ |
- |
|
Huffman, Madison L (8)
|
NCCVAC |
27.69 |
NS |
- |