| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Simpson, Ava G (9)
|
NCSAIL |
|
40.28 |
- |
| 2 |
Daniell, Kaylee F (9)
|
NCMAC |
46.90 |
40.71 |
-6.19 |
| 3 |
Bollenbacher, Heidi F (9)
|
NCMAC |
46.78 |
41.70 |
-5.08 |
| 4 |
Reale, Kailee A (9)
|
NCMAC |
|
42.48 |
- |
| 5 |
Argenta, Amalia A (9)
|
NCMAC |
|
44.04 |
- |
| 6 |
Holden, Ainsley M (9)
|
NCMAC |
47.05 |
44.21 |
-2.84 |
| 7 |
Volz, Jessie L (9)
|
NCSAIL |
44.94 |
44.61 |
-0.33 |
| 8 |
O'Dell, Sarah G (9)
|
NCMAC |
47.74 |
44.74 |
-3.00 |
| 9 |
Graham, Gabriella F (9)
|
NCMAC |
41.28 |
44.84 |
3.56 |
| 10 |
Galvin, Prema R (9)
|
NCMAC |
|
45.27 |
- |
| 11 |
Cox, Madelyn G (9)
|
NCSAIL |
48.81 |
46.54 |
-2.27 |
| 12 |
Eller, Kendall E (9)
|
NCMAC |
|
46.80 |
- |
| 13 |
Royal, Emilia I (9)
|
NCMAC |
|
47.35 |
- |
| 14 |
Ricciardi, Giovanna R (9)
|
NCMAC |
44.96 |
47.41 |
2.45 |
| 15 |
Glassner, Elyse T (9)
|
NCMAC |
49.20 |
47.54 |
-1.66 |
| 16 |
Augustyn, Carly O (9)
|
NCSAIL |
52.11 |
47.97 |
-4.14 |
| 17 |
Bass, Lillian C (9)
|
NCMAC |
|
48.03 |
- |
| 18 |
Bahr, Sophia K (9)
|
NCMAC |
|
49.05 |
- |
| 19 |
Desciscio, Annie M (9)
|
NCSAIL |
|
49.55 |
- |
| 20 |
Lindsay, Ann M (9)
|
NCMAC |
47.33 |
50.33 |
3.00 |
| 21 |
Connors, Emily R (9)
|
NCMAC |
50.43 |
51.61 |
1.18 |
| 22 |
Riddle, Taylor M (9)
|
NCMAC |
|
52.44 |
- |
| 23 |
McGuire, Georgia G (9)
|
NCMAC |
|
55.60 |
- |
| 24 |
Scott, Meredith E (9)
|
NCUN |
|
58.60 |
- |