| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hohm, Nathaniel J (10)
|
NCATOM |
38.02 |
40.16 |
2.14 |
| 2 |
Henage, Charles C (10)
|
NCWA |
48.30 |
41.86 |
-6.44 |
| 3 |
Guenther, Daniel B (10)
|
NCATOM |
45.10 |
45.08 |
-0.02 |
| 4 |
Davis, Charles W (10)
|
NCATOM |
46.89 |
45.67 |
-1.22 |
| 5 |
Spicer, Zachary W (10)
|
NCATOM |
46.27 |
47.17 |
0.90 |
| 6 |
Tabor, Beckett C (10)
|
NCGG |
48.03 |
48.36 |
0.33 |
| 7 |
Mogray, Joseph M (10)
|
NCCVAC |
47.34 |
48.43 |
1.09 |
| 8 |
Piscitelli, Gabriel M (10)
|
NCATOM |
48.08 |
48.78 |
0.70 |
| 9 |
Patton III, Kirk L (10)
|
NCATOM |
48.16 |
48.93 |
0.77 |
| 10 |
Kennedy, Patrick M (10)
|
NCATOM |
|
49.32 |
- |
| 11 |
Herger, Wyatt K (10)
|
NCATOM |
51.71 |
49.66 |
-2.05 |
| 12 |
Ashri, Nikhil (10)
|
NCGG |
49.65 |
49.68 |
0.03 |
| 13 |
Kihneman, Henry P (9)
|
NCCVAC |
48.79 |
50.18 |
1.39 |
| 14 |
Lindquist, Corbin J (10)
|
NCGG |
50.12 |
50.78 |
0.66 |
| 15 |
Smith, Gunner L (9)
|
NCCVAC |
52.09 |
51.18 |
-0.91 |
| 16 |
Black, Daniel R (10)
|
NCRCST |
51.86 |
52.60 |
0.74 |
| 17 |
Akins, Liam K (10)
|
NCATOM |
53.59 |
52.92 |
-0.67 |
| 18 |
Thomas, Robert P (10)
|
NCATOM |
58.03 |
58.03 |
0.00 |
| 19 |
Cline, Cole R (10)
|
NCCVAC |
|
1:01.55 |
- |
| 20 |
Esser, William L (10)
|
NCBAD |
|
1:02.88 |
- |
|
Olguin-Abreu, David F (9)
|
NCCVAC |
1:19.54 |
DQ |
- |
|
Michaud, Phoenix R (10)
|
NCCVAC |
1:12.75 |
DQ |
- |