| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Simpson, Ava G (9)
|
NCSAIL |
|
44.40 |
- |
| 2 |
Reale, Kailee A (9)
|
NCMAC |
|
46.67 |
- |
| 3 |
Bollenbacher, Heidi F (9)
|
NCMAC |
53.36 |
47.37 |
-5.99 |
| 4 |
Daniell, Kaylee F (9)
|
NCMAC |
53.22 |
47.53 |
-5.69 |
| 5 |
Eller, Kendall E (9)
|
NCMAC |
|
47.86 |
- |
| 6 |
Bahr, Sophia K (9)
|
NCMAC |
|
50.44 |
- |
| 7 |
Argenta, Amalia A (9)
|
NCMAC |
|
50.45 |
- |
| 8 |
Galvin, Prema R (9)
|
NCMAC |
|
50.63 |
- |
| 9 |
Cox, Madelyn G (9)
|
NCSAIL |
58.02 |
51.43 |
-6.59 |
| 9 |
O'Dell, Sarah G (9)
|
NCMAC |
55.77 |
51.43 |
-4.34 |
| 11 |
Holden, Ainsley M (9)
|
NCMAC |
55.80 |
51.78 |
-4.02 |
| 12 |
Ricciardi, Giovanna R (9)
|
NCMAC |
55.15 |
53.19 |
-1.96 |
| 13 |
Connors, Emily R (9)
|
NCMAC |
54.95 |
53.39 |
-1.56 |
| 14 |
Bass, Lillian C (9)
|
NCMAC |
|
53.93 |
- |
| 15 |
Royal, Emilia I (9)
|
NCMAC |
|
54.78 |
- |
| 16 |
Volz, Jessie L (9)
|
NCSAIL |
54.45 |
55.19 |
0.74 |
| 17 |
Lindsay, Ann M (9)
|
NCMAC |
59.33 |
55.42 |
-3.91 |
| 18 |
Glassner, Elyse T (9)
|
NCMAC |
1:01.71 |
56.68 |
-5.03 |
| 19 |
Augustyn, Carly O (9)
|
NCSAIL |
1:01.21 |
58.48 |
-2.73 |
| 20 |
Desciscio, Annie M (9)
|
NCSAIL |
|
59.47 |
- |
| 21 |
McGuire, Georgia G (9)
|
NCMAC |
|
1:02.70 |
- |
| 22 |
Riddle, Taylor M (9)
|
NCMAC |
|
1:06.46 |
- |
| 23 |
Scott, Meredith E (9)
|
NCUN |
|
1:08.75 |
- |