| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Petroff, Behrett B (10)
|
NCWOW |
38.79 |
38.19 |
-0.60 |
| 2 |
Strupe, Fredric J (10)
|
NCMSA |
38.85 |
39.47 |
0.62 |
| 3 |
Burke, Trenton G (10)
|
NCMSA |
|
42.62 |
- |
| 4 |
Roman, Vlad M (10)
|
NCMAC |
44.60 |
43.57 |
-1.03 |
| 5 |
Cagle, Griffin T (10)
|
NCMAC |
43.97 |
44.06 |
0.09 |
| 6 |
Muir, Vaughn K (10)
|
NCEAC |
45.64 |
44.33 |
-1.31 |
| 7 |
Chen, Lucas S (10)
|
NCMAC |
47.36 |
44.81 |
-2.55 |
| 8 |
Kroh, Jackson E (10)
|
NCMAC |
48.57 |
46.38 |
-2.19 |
| 9 |
Tysinger, Jacob D (10)
|
NCSAC |
46.88 |
46.54 |
-0.34 |
| 10 |
MacGregor, Jackson D (10)
|
NCATOM |
49.28 |
47.66 |
-1.62 |
| 11 |
Davis, Tyler C (10)
|
NCATOM |
51.13 |
47.81 |
-3.32 |
| 12 |
Vandenstockt, Andrew M (10)
|
NCMAC |
47.89 |
48.49 |
0.60 |
| 13 |
Harding, Cullan W (10)
|
NCMAC |
48.24 |
48.70 |
0.46 |
| 14 |
Hetzel, Samuel J (10)
|
NCMAC |
50.04 |
49.39 |
-0.65 |
| 15 |
Fazen, Cooper J (10)
|
NCMSA |
50.25 |
50.55 |
0.30 |
|
Truskowski, Logan R (10)
|
NCEAC |
40.62 |
DQ |
- |
|
Puri, Varun V (10)
|
NCATOM |
45.78 |
NS |
- |