| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Horn, Caitlin S (12)
|
NCEAC |
2:18.24 |
2:19.52 |
1.28 |
| 2 |
Schart, Halle C (12)
|
SCSCSC |
2:23.45 |
2:20.96 |
-2.49 |
| 3 |
Green, Katharine H (12)
|
SCSCSC |
2:26.30 |
2:26.84 |
0.54 |
| 4 |
Lightfoot, Illyanna J (12)
|
SCSCSC |
2:31.63 |
2:28.05 |
-3.58 |
| 5 |
Kraft, Samantha (12)
|
SCSCSC |
2:29.33 |
2:28.45 |
-0.88 |
| 6 |
Cruz, Agnes E (12)
|
NCEAC |
2:23.69 |
2:28.95 |
5.26 |
| 7 |
Hill, Caroline R (11)
|
SCSCSC |
2:32.71 |
2:29.08 |
-3.63 |
| 8 |
Bednar, Savega K (11)
|
SCSCSC |
2:32.48 |
2:29.52 |
-2.96 |
| 9 |
Oxrider, Anne Gray (12)
|
NCATOM |
2:34.55 |
2:31.06 |
-3.49 |
| 10 |
Plaza, Sofia M (11)
|
NCATOM |
2:39.66 |
2:32.21 |
-7.45 |
| 11 |
Daniels, Grace O (12)
|
NCATOM |
2:35.61 |
2:32.43 |
-3.18 |
| 12 |
Bailey, Leigh A (11)
|
NCATOM |
2:39.99 |
2:34.33 |
-5.66 |
| 13 |
Duckworth, Heather M (12)
|
NCEAC |
2:31.69 |
2:34.59 |
2.90 |
| 14 |
Blackstock, Mary M (12)
|
NCTEAM |
2:45.91 |
2:37.27 |
-8.64 |
| 15 |
Kozek, Teagan G (12)
|
NCTEAM |
2:59.99 |
2:39.20 |
-20.79 |
| 16 |
Lee, Emma K (10)
|
NCMSA |
3:15.00 |
2:39.65 |
-35.35 |
| 17 |
Darling, Cate P (12)
|
NCATOM |
2:42.77 |
2:39.93 |
-2.84 |
| 18 |
Haines, Madison A (12)
|
NCEAC |
2:40.07 |
2:42.44 |
2.37 |
| 19 |
Cagle, Margaret N (10)
|
SCSCSC |
2:40.60 |
2:44.34 |
3.74 |
| 20 |
Watson, Lily G (11)
|
GAOAC |
2:46.77 |
2:44.61 |
-2.16 |
| 21 |
Maher, Maeve C (11)
|
SCSCSC |
2:51.51 |
2:48.26 |
-3.25 |
| 22 |
Thomas, Sarah K (12)
|
NCATOM |
2:50.90 |
2:50.83 |
-0.07 |
| 23 |
White, Ehnaya K (11)
|
NCQCD |
|
2:52.69 |
- |
| 24 |
Austin, Whitney E (10)
|
NCTEAM |
|
3:14.29 |
- |
| 25 |
Kreth, Rachel O (10)
|
NCTEAM |
|
3:28.87 |
- |
|
Johnson, Claire L (10)
|
NCNSS |
3:23.37 |
DQ |
- |