| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wolk, Audrey J (12)
|
NCNCAC |
2:39.91 |
2:38.00 |
-1.91 |
| 2 |
Vassil, Avery L (11)
|
NCMAC |
2:46.55 |
2:43.59 |
-2.96 |
| 3 |
Burton, Lillian H (12)
|
NCNCAC |
2:41.78 |
2:45.71 |
3.93 |
| 4 |
Wolk, Isabel H (11)
|
NCNCAC |
|
2:45.97 |
- |
| 5 |
Hampton, Jenna M (12)
|
NCMSA |
2:49.26 |
2:47.29 |
-1.97 |
| 6 |
Su, Alicia (11)
|
NCNCAC |
3:07.99 |
2:51.01 |
-16.98 |
| 7 |
Luo, Grace L (11)
|
NCNCAC |
3:07.99 |
2:51.58 |
-16.41 |
| 8 |
Sherrill, Raegan E (12)
|
NCMSA |
2:56.24 |
2:54.29 |
-1.95 |
| 9 |
Kim, Kenzie A (11)
|
NCNCAC |
|
2:58.53 |
- |
| 10 |
Giovanello, Aurora J (11)
|
NCNCAC |
3:07.99 |
3:01.74 |
-6.25 |
| 11 |
Doucher, Maya L (11)
|
SCSWIM |
3:19.97 |
3:07.96 |
-12.01 |
| 12 |
Presson, Sadie L (12)
|
NCTEAM |
3:14.19 |
3:10.63 |
-3.56 |
|
Alessandrini, Isabella J (12)
|
NCMSA |
3:14.99 |
DQ |
- |
|
Leckner, Logan A (12)
|
NCMAC |
|
NS |
- |