| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Beckwith, Molly N (16)
|
VAGATR |
52.41 |
55.15 |
2.74 |
| 2 |
Nicholson, Lucille I (15)
|
VACA-Y |
55.00 |
55.36 |
0.36 |
| 3 |
Fix, Cayden M (17)
|
VACA-Y |
54.54 |
56.23 |
1.69 |
| 4 |
Wells, Sara T (15)
|
VACA-Y |
55.43 |
57.18 |
1.75 |
| 5 |
Hoang, Angelina J (15)
|
VACA-Y |
57.75 |
58.01 |
0.26 |
| 6 |
Gorby, Tessa L (15)
|
VAGATR |
55.08 |
58.61 |
3.53 |
| 7 |
Hines, Nelle L (16)
|
VABASS |
56.06 |
58.88 |
2.82 |
| 8 |
Breeden, Emily F (17)
|
VACA-Y |
54.56 |
59.02 |
4.46 |
| 9 |
Gahlmann, Kylin E (15)
|
VACA-Y |
59.19 |
1:01.90 |
2.71 |
| 10 |
King, Faith V (15)
|
VAGATR |
1:20.09 |
1:14.85 |
-5.24 |
|
Cave, Bella G (17)
|
VABASS |
|
NS |
- |
|
Samuels, Charlotte G (17)
|
VABASS |
54.74 |
NS |
- |
|
Whitman, Ashlyn E (16)
|
VAGATR |
1:05.29 |
NS |
- |